Synaptic AMPA receptor subunit trafficking is independent of the C terminus in the GluR2-lacking mouse

Proceedings of the National Academy of Sciences of the United States of America
Sandip PanickerRoger A Nicoll

Abstract

Glutamate is the primary excitatory neurotransmitter in the brain, and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) type glutamate receptors mediate most fast synaptic transmission. AMPA receptors are tetrameric assemblies composed from four possible subunits (GluR1-4). In hippocampal pyramidal cells, AMPA receptors are heteromeric receptors containing the GluR2 subunit and either GluR1 or GluR3. It is generally accepted that the trafficking of GluR1/GluR2 receptors to synapses requires activity, whereas GluR2/GluR3 receptors traffic constitutively. It has been suggested that the trafficking is governed by the cytoplasmic C termini of the subunits. Because the basis for this theory relied on the introduction of unnatural, homomeric, calcium-permeable AMPA receptors, we have used the GluR2(-/-) knock out mouse to determine whether the expression of mutated forms of GluR2 can rescue WT synaptic responses. We find that GluR2, lacking its entire C terminus, or a GluR2 chimera containing the C terminus of GluR1, is capable of trafficking to the synapse in the absence of activity. These findings suggest that the GluR2 C terminus is not required for GluR2 synaptic insertion.

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Citations

Mar 1, 2012·Journal of Molecular Neuroscience : MN·Juan ChengChen Zhang
Jun 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sabine M SchmidMichael Hollmann
May 22, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Melanie A GaineyGina G Turrigiano
Mar 31, 2010·The Journal of Physiology·Michelle R EmondDaniel V Madison
Jul 20, 2011·The Journal of Physiology·Adam J GrangerRoger A Nicoll
Mar 27, 2009·Journal of Cellular and Molecular Medicine·Michael FöllerFlorian Lang
Jul 1, 2009·Microscopy Research and Technique·Armin H Seidl, Edwin W Rubel
Feb 17, 2009·Neuron·Helmut W Kessels, Roberto Malinow
May 24, 2008·Neuron·Thomas M Newpher, Michael D Ehlers
Sep 8, 2010·Molecular and Cellular Neurosciences·Patricio Opazo, Daniel Choquet
Nov 4, 2009·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Seung Hyun JeunKi-Wug Sung
Jun 6, 2021·Neuropharmacology·Radu Gugustea, Zhengping Jia
Sep 10, 2021·Frontiers in Molecular Neuroscience·Ali HarbRalf Mohrmann

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