Synaptic and extrasynaptic localization of brain-derived neurotrophic factor and the tyrosine kinase B receptor in cultured hippocampal neurons

The Journal of Comparative Neurology
Catherine Croft SwanwickJaideep Kapur

Abstract

Brain-derived neurotrophic factor (BDNF) regulates synapses, but the distribution of BDNF and its receptor TrkB relative to the location of glutamatergic and gamma-aminobutyric acidergic (GABAergic) synapses is presently unknown. Immunocytochemistry was performed in primary hippocampal neuron cultures to determine whether BDNF and TrkB are preferentially localized to excitatory or inhibitory markers at 7, 14, and 21 days in vitro (DIV). Glutamatergic sites were localized with vesicular glutamate transporter type 1 (VGLUT1) as presynaptic marker and the NR1 subunit of the NMDA receptor and the GluR1 subunit of the AMPA receptor as receptor markers. GABAergic sites were labeled with the 65-kDa isoform of glutamic acid decarboxylase (GAD-65) as presynaptic marker and the gamma2 subunit of the GABAA receptor as receptor marker. During development, <30% of BDNF punctae and TrkB clusters were localized to glutamatergic and GABAergic markers. Because their rates of colocalization did not change from 7 to 21 DIV, this study details the distribution of BDNF and TrkB at 14 DIV. BDNF was preferentially colocalized with glutamatergic markers VGLUT1 and NR1 ( approximately 30% each). TrkB was also relatively highly colocalized with VGLUT1 a...Continue Reading

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Citations

Jan 11, 2005·Neurotoxicity Research·Huanyu DouHoward E Gendelman
Sep 25, 2009·Experimental Brain Research·Kurt GottmannVolkmar Lessmann
Apr 26, 2012·Brain Structure & Function·Zhi-Gang YaoChuan Qin
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kang ZhengBai Lu
Aug 2, 2005·Epilepsy Currents·Catherine Croft Swanwick, Jaideep Kapur
Jul 20, 2007·Journal of Cell Science·Carlene D MooreAnne Theibert
Oct 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Prabhu RamamoorthyMatthew D Whim
Mar 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Howard P GoodkinJaideep Kapur
Dec 9, 2014·Frontiers in Biology·Li-Ya Qiao
Jun 16, 2009·Neuroscience Research·Volkmar Lessmann, Tanja Brigadski
Jan 27, 2009·Neuroscience·I MeloniA Renieri
Jun 17, 2011·Alcoholism, Clinical and Experimental Research·Tara A LindsleyElizabeth A Ruggiero
Feb 4, 2010·The Journal of Comparative Neurology·Angeliki Maria NikolakopoulouSusana Cohen-Cory
Feb 27, 2010·Developmental Neurobiology·Susana Cohen-CorySonya Marshak
Aug 25, 2009·Brain Research·Santina ZanelliJaideep Kapur
Dec 7, 2005·Molecular and Cellular Neurosciences·Catherine Croft SwanwickJaideep Kapur
Jun 4, 2013·Neuropharmacology·Maija L Castrén, Eero Castrén
Jun 25, 2013·Neuropharmacology·Elke EdelmannTanja Brigadski
Aug 26, 2016·Biophysical Journal·Benjamin J RobinsonBethe A Scalettar
Sep 5, 2007·The European Journal of Neuroscience·C A BlizzardT C Dickson
Nov 1, 2018·Brain Structure & Function·Julia L HillKeri Martinowich
Feb 25, 2006·The Journal of Comparative Neurology·Catherine Croft SwanwickJaideep Kapur

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