Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster

The Journal of Comparative Neurology
Jürgen RybakBill S Hansson

Abstract

In Drosophila melanogaster olfactory sensory neurons (OSNs) establish synapses with projection neurons (PNs) and local interneurons within antennal lobe (AL) glomeruli. Substantial knowledge regarding this circuitry has been obtained by functional studies, whereas ultrastructural evidence of synaptic contacts is scarce. To fill this gap, we studied serial sections of three glomeruli using electron microscopy. Ectopic expression of a membrane-bound peroxidase allowed us to map synaptic sites along PN dendrites. Our data prove for the first time that each of the three major types of AL neurons is both pre- and postsynaptic to the other two types, as previously indicated by functional studies. PN dendrites carry a large proportion of output synapses, with approximately one output per every three input synapses. Detailed reconstructions of PN dendrites showed that these synapses are distributed unevenly, with input and output sites partially segregated along a proximal-distal gradient and the thinnest branches carrying solely input synapses. Moreover, our data indicate synapse clustering, as we found evidence of dendritic tiling of PN dendrites. PN output synapses exhibited T-shaped presynaptic densities, mostly arranged as tetrads...Continue Reading

References

May 29, 1992·Science·B S HanssonC Löfstedt
Mar 8, 1991·The Journal of Comparative Neurology·I A Meinertzhagen, S D O'Neil
Oct 1, 1990·Cell and Tissue Research·R F StockerK F Fischbach
Jun 1, 1969·Journal of Ultrastructure Research·O Trujillo-Cenóz
Feb 15, 1993·Microscopy Research and Technique·J Boeckh, L P Tolbert
Sep 2, 1996·The Journal of Comparative Neurology·B Leitch, G Laurent
Oct 21, 1996·Journal of Neuroscience Methods·I A Meinertzhagen
Jan 1, 1997·Annual Review of Neuroscience·J G Hildebrand, G M Shepherd
Feb 1, 1997·The European Journal of Neuroscience·J Rybak, I A Meinertzhagen
May 1, 1997·Journal of Neurobiology·R F StockerJ S de Belle
Apr 23, 1999·The Journal of Comparative Neurology·H J KasowskiC A Greer
Aug 16, 2000·Cell·L B VosshallR Axel
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·A DidierP M Lledo
Jun 8, 2001·Neuron·M de BruyneJ R Carlson
Aug 9, 2001·The Journal of Comparative Neurology·R AbelR Menzel
Feb 13, 2002·Current Biology : CB·Karlheinz ReinMartin Heisenberg
Feb 28, 2002·Current Opinion in Neurobiology·Gregory S X E JefferisLiqun Luo
Aug 27, 2002·Annual Review of Entomology·Lynne A Oland, Leslie P Tolbert
Jan 9, 2003·The Journal of Experimental Biology·Marcus C StensmyrBill S Hansson
Mar 17, 2004·Brain Research. Gene Expression Patterns·P M Salvaterra, T Kitamoto
Apr 7, 2004·Neuron·Thomas Hummel, S Lawrence Zipursky
Apr 16, 2004·Current Biology : CB·Ryan J WattsLiqun Luo
Jun 7, 2005·Cell·Petra StockingerBarry J Dickson
Jun 21, 2005·Current Biology : CB·A Aldo FaisalSimon B Laughlin
Sep 6, 2005·Current Biology : CB·Africa CoutoBarry J Dickson
Sep 6, 2005·Current Biology : CB·Elane Fishilevich, Leslie B Vosshall
Oct 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rachel I Wilson, Gilles Laurent
Nov 5, 2005·Neuron·Barry W Ache, Janet M Young
Mar 28, 2006·The Journal of Comparative Neurology·Christina KelberChristoph Johannes Kleineidam

❮ Previous
Next ❯

Citations

May 14, 2016·ELife·Matthew E BerckAlbert Cardona
Mar 29, 2016·The Journal of Comparative Neurology·Xin-Cheng ZhaoBente G Berg
Nov 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Sudeshna DasSilke Sachse
Feb 6, 2020·The Journal of Comparative Neurology·Hyunsoo KimAzusa Kamikouchi
Aug 8, 2017·ELife·Ivan LarderetSimon G Sprecher
Apr 11, 2018·Frontiers in Cellular Neuroscience·Lydia GruberRafael Cantera
Nov 4, 2020·Nature Methods·Sebastian CacheroGregory S X E Jefferis
May 26, 2021·ELife·Philipp SchlegelGregory S X E Jefferis
Nov 30, 2021·The Journal of Comparative Neurology·Steffen HarzschBill S Hansson

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
transgenic
transmission electron microscopy
dissection
confocal microscopy
fluorescence microscopy
light microscopy

Key Resources (RRID) Mentioned

BDSC_30026
BDSC_32116
BDSC_26818
BDSC_9951
nlx_151924

Software Mentioned

GRASP
draw
Adobe
Amira
Acrobat Reader
Acrobat
ImageJ
Adobe Photoshop CS
Adobe 3D reviewer
mlALT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.