Synaptic Conductance Estimates of the Connection Between Local Inhibitor Interneurons and Pyramidal Neurons in Layer 2/3 of a Cortical Column

Cerebral Cortex
Jochen H O HoffmannMoritz Helmstaedter

Abstract

Stimulation of a principal whisker yields sparse action potential (AP) spiking in layer 2/3 (L2/3) pyramidal neurons in a cortical column of rat barrel cortex. The low AP rates in pyramidal neurons could be explained by activation of interneurons in L2/3 providing inhibition onto L2/3 pyramidal neurons. L2/3 interneurons classified as local inhibitors based on their axonal projection in the same column were reported to receive strong excitatory input from spiny neurons in L4, which are also the main source of the excitatory input to L2/3 pyramidal neurons. Here, we investigated the remaining synaptic connection in this intracolumnar microcircuit. We found strong and reliable inhibitory synaptic transmission between intracolumnar L2/3 local-inhibitor-to-L2/3 pyramidal neuron pairs [inhibitory postsynaptic potential (IPSP) amplitude -0.88 ± 0.67 mV]. On average, 6.2 ± 2 synaptic contacts were made by L2/3 local inhibitors onto L2/3 pyramidal neurons at 107 ± 64 µm path distance from the pyramidal neuron soma, thus overlapping with the distribution of synaptic contacts from L4 spiny neurons onto L2/3 pyramidal neurons (67 ± 34 µm). Finally, using compartmental simulations, we determined the synaptic conductance per synaptic contac...Continue Reading

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Citations

Oct 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Robert EggerJason N D Kerr
Jul 15, 2017·ELife·Benedikt StafflerMoritz Helmstaedter
Sep 30, 2017·Nature·Helene SchmidtMoritz Helmstaedter
Apr 26, 2019·PLoS Computational Biology·Renato Duarte, Abigail Morrison
Oct 4, 2019·Nature Communications·Ryota KobayashiShigeru Shinomoto
Jun 14, 2019·Frontiers in Neuroinformatics·Roozbeh FarhoodiKonrad Paul Kording

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Methods Mentioned

BETA
light microscopy
electron microscopy

Software Mentioned

Neurolucida
NEURON

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Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.