Synaptic connections of PDF-immunoreactive lateral neurons projecting to the dorsal protocerebrum of Drosophila melanogaster

The Journal of Comparative Neurology
Kouji Yasuyama, Ian A Meinertzhagen

Abstract

Recent studies in Drosophila melanogaster indicate that the neuropeptide pigment-dispersing factor (PDF) is an important output signal from a set of major clock neurons, s-LN(v)s (small ventral lateral neurons), which transmit the circadian phase to subsets of other clock neurons, DNs (dorsal neurons). Both s-LN(v)s and DNs have fiber projections to the dorsal protocerebrum of the brain, so that this area is a conspicuous locus for coupling between different subsets of clock neurons. To unravel the neural circuits underlying the fly's circadian rhythms, we examined the detailed subcellular morphology of the PDF-positive fibers of the s-LN(v)s in the dorsal protocerebrum, focusing on their synaptic connections, using preembedding immunoelectron microscopy. To examine the distribution of synapses, we also reconstructed the three-dimensional morphology of PDF-positive varicosities from fiber profiles in the dorsal protocerebrum. The varicosities contained large dense-core vesicles (DCVs), and also numerous small clear vesicles, forming divergent output synapses onto unlabeled neurites. The DCVs apparently dock at nonsynaptic sites, suggesting their nonsynaptic release. In addition, a 3D reconstruction revealed the presence of inpu...Continue Reading

References

Apr 8, 1998·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·C Helfrich-Förster
Mar 29, 2002·The Journal of Comparative Neurology·Kouji YasuyamaFriedrich-Wilhelm Schürmann
Sep 11, 2003·Microscopy Research and Technique·Charlotte Helfrich-Förster
Sep 17, 2003·PLoS Biology·Ying PengMichael Rosbash
Sep 10, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yiing LinPaul H Taghert
Apr 9, 2005·Methods in Enzymology·Jeffrey C Hall
Aug 25, 2005·Journal of Neurobiology·Yasutaka HamasakaDick R Nässel
Dec 2, 2005·The Journal of Comparative Neurology·Yasutaka Hamasaka, Dick R Nässel
Dec 31, 2005·Seminars in Cell & Developmental Biology·Andreas Prokop, Ian A Meinertzhagen
Jan 13, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael N NitabachTodd C Holmes
Jan 18, 2006·Behavioural Processes·Dennis C Chang
Mar 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dirk RiegerCharlotte Helfrich-Förster
Jul 21, 2006·The Journal of Comparative Neurology·Orie Thomas ShaferPaul H Taghert
Nov 14, 2006·The Journal of Comparative Neurology·Charlotte Helfrich-FörsterPaul Taghert
Nov 17, 2006·Journal of Biological Rhythms·Paul H Taghert, Orie T Shafer
Jun 10, 2008·The Journal of Comparative Neurology·Shin-Ya TakemuraIan A Meinertzhagen

❮ Previous
Next ❯

Citations

Mar 3, 2011·Cellular and Molecular Life Sciences : CMLS·Johannes StraussHeinrich Dircksen
Aug 25, 2015·FEBS Letters·Esteban J Beckwith, M Fernanda Ceriani
Dec 17, 2014·The Journal of Comparative Neurology·Esteban J Beckwith, M Fernanda Ceriani
Aug 27, 2014·Current Biology : CB·E Axel GorostizaMaría Fernanda Ceriani
Jun 4, 2013·Neuroscience·N I MuraroM F Ceriani
Apr 11, 2013·Fly·Sheila K Pirooznia, Felice Elefant
Oct 25, 2011·Current Biology : CB·Ana Depetris-ChauvinMaría Fernanda Ceriani
Oct 9, 2012·Neuron·Paul H Taghert, Michael N Nitabach
Feb 11, 2018·The Journal of Comparative Neurology·Mareike SelchoKouji Yasuyama
Jul 31, 2018·The European Journal of Neuroscience·Anna N King, Amita Sehgal
Mar 23, 2019·JMIR MHealth and UHealth·Kerina Helen JonesDavid Vincent Ford
Oct 1, 2018·The European Journal of Neuroscience·Aliza K De Nobrega, Lisa C Lyons
Aug 8, 2017·ELife·Ivan LarderetSimon G Sprecher
Jul 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Shinsuke FujiiHubert Amrein
Mar 2, 2021·Journal of Biological Rhythms·Myra AhmadDeniz Top
May 11, 2020·Current Biology : CB·Maria P FernandezOrie T Shafer
Aug 5, 2020·Current Biology : CB·François Rouyer, Abhishek Chatterjee
Aug 12, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Binbin WuZhangwu Zhao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.