Synaptic mitochondria: a brain mitochondria cluster with a specific proteome

Journal of Proteomics
Katalin VölgyiArpád Dobolyi

Abstract

The synapse is a particularly important compartment of neurons. To reveal its molecular characteristics we isolated whole brain synaptic (sMito) and non-synaptic mitochondria (nsMito) from the mouse brain with purity validated by electron microscopy and fluorescence activated cell analysis and sorting. Two-dimensional differential gel electrophoresis and mass spectrometry based proteomics revealed 22 proteins with significantly higher and 34 proteins with significantly lower levels in sMito compared to nsMito. Expression differences in some oxidative stress related proteins, such as superoxide dismutase [Mn] (Sod2) and complement component 1Q subcomponent-binding protein (C1qbp), as well as some tricarboxylic acid cycle proteins, including isocitrate dehydrogenase subunit alpha (Idh3a) and ATP-forming β subunit of succinyl-CoA ligase (SuclA2), were verified by Western blot, the latter two also by immunohistochemistry. The data suggest altered tricarboxylic acid metabolism in energy supply of synapse while the marked differences in Sod2 and C1qbp support high sensitivity of synapses to oxidative stress. Further functional clustering demonstrated that proteins with higher synaptic levels are involved in synaptic transmission, lac...Continue Reading

References

Apr 30, 1986·Brain Research·P R DunkleyJ A Rostas
May 1, 1994·Renal Physiology and Biochemistry·P J MagistrettiJ L Martin
Aug 31, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Magistretti, L Pellerin
Nov 22, 2000·Brain Research. Brain Research Reviews·A Ames
Jun 8, 2001·Journal of Neuroscience Research·A E GioioB B Kaplan
Jun 21, 2001·Molecular Neurobiology·B Hassel
May 2, 2002·The European Journal of Neuroscience·Alexei P KudinWolfram S Kunz
Jul 20, 2002·Trends in Neurosciences·Antonio GiudittaEdward Koenig
Jul 21, 2004·Journal of Neuroscience Methods·Maile R BrownOswald Steward
Aug 18, 2004·Progress in Neurobiology·Maria ErecinskaIan A Silver
Jan 28, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicola J AllenDavid Attwell
Apr 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Alessandro RivaCharles Hoppel
Aug 2, 2005·Neuron·Peter J Hollenbeck
Nov 25, 2005·Journal of Cell Science·Peter J Hollenbeck, William M Saxton
Mar 7, 2006·The Journal of Biological Chemistry·Maile R BrownJames W Geddes
Oct 5, 2006·Cell·Michael A Sutton, Erin M Schuman
Oct 21, 2006·Biochemical Society Transactions·R J KittelM Heckmann
Jul 10, 2007·Cellular and Molecular Neurobiology·Mi HilleforsBarry B Kaplan
Jul 5, 2008·Nature Protocols·Neil R Sims, Michelle F Anderson
Dec 17, 2008·Neuron·Mark P MattsonAiwu Cheng
May 30, 2009·Free Radical Biology & Medicine·Lu Miao, Daret K St Clair
Aug 26, 2009·Cell·Agnieszka ChacinskaNikolaus Pfanner
Nov 3, 2009·Brain Research Reviews·L Felipe Barros, Joachim W Deitmer
Dec 17, 2009·Trends in Cell Biology·Andrew F MacAskill, Josef T Kittler
Dec 18, 2009·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Eva M SzegoKatalin A Kékesi
May 20, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthias T WyssBruno Weber
Apr 19, 2012·Nature Reviews. Neuroscience·Husseini ManjiGuang Chen
Jun 30, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Catherine N HallDavid Attwell
Jan 8, 2013·Neuropathology and Applied Neurobiology·T H Gillingwater, T M Wishart
Jul 11, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Vivek TiwariAnant B Patel
Aug 24, 2013·Frontiers in Endocrinology·Arne SchousboeHelle S Waagepetersen

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Citations

Aug 25, 2015·American Journal of Human Genetics·Akemi J TanakaWendy K Chung
Feb 15, 2017·Reviews in the Neurosciences·Sergei V FedorovichLudmila V Puchkova
Jan 5, 2019·Biological Reviews of the Cambridge Philosophical Society·Alessandra DonatoMassimo A Hilliard
Apr 14, 2017·EMBO Reports·Vidhya RangarajuErin M Schuman
Jun 18, 2017·Molecular Neurobiology·Katalin VölgyiArpád Dobolyi
Feb 26, 2016·Molecular Neurobiology·Katalin VölgyiKatalin Adrienna Kékesi
Jan 1, 2017·Neurochemical Research·Andrzej SzutowiczJoanna Klimaszewska-Łata
Sep 16, 2017·Neurochemical Research·Carina de Souza MotaLetícia Ferreira Pettenuzzo
Mar 16, 2019·Scientific Reports·János BarnaÁrpád Dobolyi
Oct 13, 2016·The Biochemical Journal·Manuel MaidornFelipe Opazo
Nov 20, 2020·Amino Acids·Péter GulyássyKatalin Adrienna Kékesi
Sep 27, 2020·Molecular and Cellular Neurosciences·Virginie RappeneauChadi Touma
Feb 2, 2021·Frontiers in Molecular Neuroscience·Magdalena CieślikAgata Adamczyk
Feb 7, 2021·International Journal of Molecular Sciences·András H LékóArpád Dobolyi
Feb 16, 2021·EMBO Reports·Roya YousefiDavid Pacheu-Grau
Jul 24, 2021·Neurobiology of Disease·Sergei V BaranovRobert M Friedlander

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