Synaptic NMDA receptors in developing mouse hippocampal neurones: functional properties and sensitivity to ifenprodil

The Journal of Physiology
E D Kirson, Y Yaari

Abstract

1. Whole-cell patch-clamp techniques were used to record pharmacologically isolated NMDA receptor-mediated EPSCs (NMDA EPSCs) from CA1 pyramidal cells (PCs) in hippocampal slices from 4-day-old to 36-week-old mice, in order to characterize developmental changes in functional properties and subunit composition of synaptic NMDA receptors. 2. During the first postnatal weeks the dendritic tree of CA1 PCs stained with biocytin increased both in size and in complexity. This was associated with an increase in amplitude of the focally evoked NMDA EPSCs recorded either in nominally Mg(2+)-free or Mg(2+)-containing saline. In adult PCs (> 5 weeks old) EPSC amplitude was 4-fold larger than in very young (up to 2 weeks old) neurones. 3. The sensitivity of NMDA EPSCs to blockade by Mg2+ did not change with age. In very young, intermediate and adult PCs the EPSC-voltage relation displayed an area of negative slope conductance at membrane potentials more negative than -30 mV. The apparent Kd values of the NMDA receptors for Mg2+ at 0 mV were 7.8 +/- 6.4, 10.4 +/- 14.1 and 6.5 +/- 4.7 mM in very young, intermediate and adult neurones, respectively. 4. The decay of the NMDA EPSC in both young and adult neurones could be described by the sum of...Continue Reading

Citations

Apr 2, 1999·The European Journal of Neuroscience·J I PalecekL Vyklick
Jul 25, 2006·Cell and Tissue Research·Georg Köhr
Oct 30, 2004·The Journal of Physiology·Anqi QianJon W Johnson
May 18, 2004·The Journal of Physiology·Natasha A LozovayaOleg A Krishtal
Feb 22, 2012·The Journal of Physiology·Elena E Bagley, Gary L Westbrook
Jan 5, 2002·The Journal of Physiology·Galya AbdrachmanovaLadislav Vyklický
Jun 7, 2011·Molecular and Cellular Neurosciences·Lucie Bard, Laurent Groc
May 31, 2011·Progress in Neurobiology·Michelle R Lyons, Anne E West
Mar 2, 2016·ELife·Shai BerlinEhud Y Isacoff
Aug 25, 2007·The Journal of Physiology·Alexander Z Harris, Diana L Pettit
Feb 23, 2012·Scientific Reports·Kimberly Moore Olsen, Morgan Sheng
Apr 29, 2005·Journal of Neurochemistry·I PaarmannM Hollmann
May 29, 2009·The European Journal of Neuroscience·Ilse RiebeEric Hanse
Mar 15, 2005·Brain Research. Developmental Brain Research·Gui-Lan YeBarbara L Trommer
Sep 12, 2006·Epilepsy Research·Susanta Bandyopadhyay, John J Hablitz
Aug 12, 2006·Neuropharmacology·Sven BerberichGeorg Köhr
Dec 24, 2013·Progress in Neurobiology·Ted Weita LaiYu Tian Wang
May 3, 2016·Journal of Neurodevelopmental Disorders·Duncan SinclairKarin E Borgmann-Winter
Feb 14, 2007·Neuron·Benjamin D PhilpotMark F Bear

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