PMID: 8606790Nov 1, 1995Paper

Synaptic transmission at single visualized hippocampal boutons

Neuropharmacology
G Liu, R W Tsien

Abstract

We have used a focal stimulation method to study neurotransmission at synapses onto hippocampal pyramidal neurons in cultures derived from neonatal rats. Single functional boutons were visualized by activity-dependent preloading with the fluorescent dye FM1-43, then focally stimulated by localized application of elevated K+/Ca2+ solution via a puffer pipette, while postsynaptic currents were recorded under whole cell voltage clamp (Liu and Tsien, 1995). This paper gives a detailed description of the main properties of this experimental system and of information it has provided about fundamental properties of hippocampal synapses. Most of the experiments focused on excitatory postsynaptic currents (EPSCs), but preliminary recordings of inhibitory events (IPSCs) are also reported here. The unitary EPSCs at individual synapses varied greatly in amplitude, but were relatively uniform in their time course. The frequency of the synaptic events was greatly reduced by lowering the external Ca2+ concentration or by application of baclofen, a GABAB receptor agonist. Frequent repetitive stimulation produced a decline in the incidence of EPSCs that was readily reversed upon rest. We attribute the decline to exhaustion of a pool of availabl...Continue Reading

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Citations

Mar 7, 1998·Journal of Neuroscience Methods·W Van der Kloot
Apr 15, 1999·Annual Review of Neuroscience·A J CochillaW J Betz
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y Goda, C F Stevens
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Mar 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yiguo ShenLouis J Ptáček
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Feb 25, 2018·Nature Communications·Kristina E KitkoQi Zhang
Jun 18, 2010·Journal of Neurophysiology·Miriam Ivenshitz, Menahem Segal
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Feb 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Chungwon ParkHyokeun Park
Jun 6, 2018·ACS Chemical Neuroscience·Claire E DelBoveQi Zhang

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