Synaptically evoked glutamate transport currents may be used to detect the expression of long-term potentiation in cerebellar culture

Journal of Neurophysiology
D J Linden

Abstract

Cerebellar long-term potentiation (LTP) is a use-dependent increase in the strength of the granule cell-Purkinje neuron synapse that occurs after brief stimulation of granule cell axons at 2-8 Hz. Previous work has shown that cerebellar LTP also may be seen when synaptic currents are evoked in granule cell-glial cell pairs in culture. This finding suggests a model in which cerebellar LTP is expressed presynaptically and therefore may be detected by either neuronal or glial postsynaptic cells. However, synaptic currents evoked in both granule cell-glial cell pairs and granule cell-Purkinje neuron pairs in culture are mediated primarily by alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptors, raising the possibility that cerebellar LTP might be expressed postsynaptically in both glial cells and Purkinje neurons in a similar manner. To address this question, glutamate transport currents were recorded in granule cell-glial cell pairs in culture by pharmacological isolation. These currents were increased by substitution of internal Cl with NO3 and were blocked by -pyrrolidine-2,4-dicarboxylate, both characteristics of the major cloned Bergmann glial cell glutamate transporter, EAAT1. After acquisition of...Continue Reading

References

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Citations

Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Varda Lev-RamRoger Y Tsien
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Varda Lev-RamRoger Y Tsien
Jan 5, 2005·Molecular Psychiatry·P García-Junco-ClementeR Fernández-Chacón
Feb 8, 2003·Journal of Neurophysiology·Angélique Bordey, Harald Sontheimer
Sep 9, 2015·Neural Plasticity·Giselle CheungNathalie Rouach
Jan 6, 2021·Progress in Neurobiology·Karim A Alkadhi

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