Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans

Genetics
Tisha BohrNeedhi Bhalla

Abstract

Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint.

Citations

Apr 3, 2001·Nature Reviews. Genetics·T Hassold, P Hunt
Aug 28, 2003·Médecine sciences : M/S·Christelle Gally, Jean-Louis Bessereau
Nov 18, 2005·Genes & Development·Florence Couteau, Monique Zetka
Feb 13, 2008·Developmental Cell·William GoodyerMonique Zetka
Jul 4, 2008·Annual Review of Cell and Developmental Biology·Needhi Bhalla, Abby F Dernburg
Apr 12, 2011·Nature Cell Biology·Katrin DanielAttila Tóth
May 3, 2011·Trends in Cell Biology·Amy J Macqueen, Andreas Hochwagen
Oct 21, 2015·The Journal of Cell Biology·Tisha BohrNeedhi Bhalla

Related Concepts

Calcinus elegans
Cyartonema elegans
Health Center
Coleonyx elegans
Chromosomal Proteins, Non-Histone
Cestrum elegans
Clarkia unguiculata
Clathrulina elegans
Cell Cycle Proteins
Htp-1 protein, C elegans

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