PMID: 589479Dec 16, 1977

Synaptosomal plasma membrane glycoproteins. II. Isolation of fucosyl-glycoproteins by affinity chromatography on the Ulex europeus lectin specific for L-fucose

Brain Research
J P ZanettaG Gombos

Abstract

After solubilization in sodium dodecyl sulphate, almost 90% of synaptosomal plasma membrane glycoproteins were separated from the bulk of synaptosomal plasma membrane proteins by sequential affinity chromatography on two immobilized lectins: concanavalin A and the Ulex europeus lectin specific for L-fucose. Four fractions were obtained and their sugar composition and electrophoretic patterns were determined. Fucosyl-glycoproteins contain more than 26% of protein and 85% of the protein-bound sugar of synaptosomal plasma membrane; hence they constitute a major class of glycoproteins in these membranes. The presence of some glucose in glycoproteins fractions obtained after affinity chromatography on the two lectins suggests that this sugar could be a structural component of some brain glycoproteins. Polyacrylamide gel electrophoresis revealed at least 28 major bands in fucosylglycoprotein fractions, and 11 in other fractions. Several of these major bands appear to contain more than one glycoprotein each. This heterogeneity appears to be mostly the result of the heterogeneity of the neuronal population in the central nervous system. Microheterogeneity of glycoprotein sugar chains and possible contamination of synaptosomal plasma me...Continue Reading

References

Apr 15, 1977·Brain Research·J P ZanettaG Gombos
Aug 13, 1976·Brain Research·R K MargolisR U Margolis
Jan 1, 1987·Methods in Enzymology·R TimplS Fujiwara
Sep 14, 1971·Biochimica Et Biophysica Acta·L S WolfeG Gombos
Apr 28, 1973·Biochimica Et Biophysica Acta·R K Margolis, R U Margolis
Dec 1, 1971·Journal of Neurochemistry·R M Grossfeld, E M Shooter
Nov 11, 1969·Biochimica Et Biophysica Acta·I Matsumoto, T Osawa

Citations

May 1, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·A WernigD Tonge
Oct 28, 1981·Biochimica Et Biophysica Acta·J P ZanettaG Vincendon
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·P W Beesley
Feb 5, 2003·Biochemical and Biophysical Research Communications·Iku KuwaharaKiyoshi Furukawa
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·P R Satish, A Surolia
Oct 26, 2005·International Journal of Biological Sciences·Kate KolesSteven P R Rose
May 3, 2008·Chemical Reviews·Heather E Murrey, Linda C Hsieh-Wilson
Mar 1, 1982·Journal of Neurochemistry·S GustavssonJ O Karlsson

Related Concepts

Chromatography, Affinity
Concanavalin A
Fucose
Glucose, (beta-D)-Isomer
Neoglycoproteins
Synaptic Membranes
Animal Lectins

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