PMID: 9175268Jan 1, 1996Paper

Synaptotagmin II expression partially rescues the growth defect of the yeast sec15 secretory mutant

Biology of the Cell
C K Damer, C E Creutz

Abstract

Synaptotagmins are a family of calcium- and phospholipid-binding proteins implicated in the function of cell exocytosis. Synaptotagmins I and II are neurally expressed proteins thought to be involved in neurotransmitter release from neurons. We have expressed rat synaptotagmin II in several Saccharomyces cerevisiae temperature-sensitive secretory mutants that are defective in Golgi to plasma membrane vesicular transport. Synaptotagmin II expression was able to partially rescue the growth defect in one particular mutant, sec15. No suppression was observed when synaptotagmin II was expressed in sec1, sec2, sec4, sec5, sec6, sec8, sec9, sec14, sec17, or sec18. Two synaptotagmin II deletion mutants were also expressed in sec15 and screened for suppression. The expression of the cytoplasmic domain of synaptotagmin alone was not able to suppress the sec15 growth defect. In addition, the expression of a synaptotagmin II fragment lacking the second half of the cytoplasmic domain including the second C2 domain did not suppress sec15. We have isolated a membrane fraction enriched in post-Golgi vesicles from a sec15 strain expressing synaptotagmin II and found that synaptotagmin II co-purifies with this fraction, suggesting that the rat s...Continue Reading

References

Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R BlockJ E Rothman
Jul 1, 1987·The Journal of Cell Biology·N C Walworth, P J Novick
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W S TrimbleR H Scheller
Aug 1, 1981·Cell·P NovickR Schekman
Oct 6, 1995·The Journal of Biological Chemistry·E R ChapmanR Jahn
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·A E TingR H Scheller
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·A W Hudson, M J Birnbaum
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·J T LittletonH J Bellen
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·B S Hilbush, J I Morgan
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M K Bennett, R H Scheller
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Mar 8, 1996·The Journal of Biological Chemistry·E R ChapmanR Jahn

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