Synaptotagmin II peptide-bead conjugate for botulinum toxin enrichment and detection in microchannels

Biosensors & Bioelectronics
Megan L FriskDavid J Beebe

Abstract

This paper reports an enrichment platform for botulinum neurotoxin type B (BoNT/B) that has been realized through the fusion of bioconjugation chemistry and microfluidics. Micrometer-sized magnetic beads were conjugated to a 22mer synthetic peptide derived from the synaptotagmin II (Syt II) neuronal protein that is specific for BoNT/B binding. Exposure to BoNT/B in buffer, whole milk and fruit juices resulted in toxin capture, which was confirmed using immunofluorescence. Peptide-modified beads were integrated into arrayed, polymeric microfluidic channels, and all assay steps, from capture to detection, were performed directly in the microchannels, thereby simplifying assay utility and increasing throughput relative to existing detection methodologies. Our sensitive microscale approach required only 7 μL of intentionally adulterated sample without any pre-processing (i.e. dilution, centrifugation, filtering), and with a "hands-on" time of only 1 h to detect 16.6 pg of BoNT/B in whole milk.

References

Mar 7, 2001·JAMA : the Journal of the American Medical Association·S S ArnonUNKNOWN Working Group on Civilian Biodefense
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Hai-Yuan ChaoHwan-Wun Liu
Apr 22, 2004·Lab on a Chip·Glenn M Walker, David J Beebe
Apr 6, 2005·Drug Discovery Today·Philipp Angenendt
Oct 20, 2005·Journal of Biomolecular Screening·Shakuntala MangruKrishna Kodukula
May 30, 2006·Toxicon : Official Journal of the International Society on Toxinology·Hongzheng MaKim D Janda
Mar 3, 2007·Lab on a Chip·Hongmei YuDavid J Beebe
Oct 23, 2008·Lab on a Chip·Megan L FriskDavid J Beebe
Jun 23, 2009·Journal of Immunological Methods·Andrew N Hoofnagle, Mark H Wener
Oct 30, 2009·Lab on a Chip·Steven SunAvraham Rasooly

❮ Previous
Next ❯

Citations

Dec 16, 2011·Analytical and Bioanalytical Chemistry·Silvia Pavan, Federico Berti
May 7, 2013·Annual Review of Biomedical Engineering·Scott BantaOren Shur
Nov 5, 2016·Toxicology and Applied Pharmacology·Oliver G Weingart, Martin J Loessner
Apr 12, 2014·Electrophoresis·Zhaowei ZhangXiaojun Feng
Oct 23, 2013·Lab on a Chip·H Cumhur Tekin, Martin A M Gijs
Apr 16, 2013·Lab on a Chip·Carlos Escobedo
Dec 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Weiwei YeMo Yang
Feb 11, 2020·Biomicrofluidics·Pablo E Guevara-PantojaGabriel A Caballero-Robledo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.