Synaptotagmin perturbs the structure of phospholipid bilayers.

Biochemistry
Victor ShahinJ Michael Edwardson

Abstract

Synaptotagmin I (syt), an integral protein of the synaptic vesicle membrane, is believed to act as a Ca2+ sensor for neuronal exocytosis. Syt's cytoplasmic domain consists largely of two C2 domains, C2A and C2B. In response to Ca2+ binding, the C2 domains interact with membranes, becoming partially embedded in the lipid bilayer. We have imaged syt C2AB in association with lipid bilayers under fluid, using AFM. As expected, binding of C2AB to bilayers required both an anionic phospholipid [phosphatidylserine (PS)] and Ca2+. C2AB associated with bilayers in the form of aggregates of varying stoichiometries, and aggregate size increased with an increase in PS content. Repeated scanning of bilayers revealed that as C2AB dissociated it left behind residual indentations in the bilayer. The mean depth of these identations was 1.81 nm, indicating that they did not span the bilayer. Individual C2 domains (C2A and C2B) also formed aggregates and produced bilayer indentations. Binding of C2AB to bilayers and the formation of indentations were significantly compromised by mutations that interfere with binding of Ca2+ to syt or reduce the positive charge on the surface of C2B. We propose that bilayer perturbation by syt might be significant...Continue Reading

References

Oct 6, 1995·The Journal of Biological Chemistry·E R ChapmanR Jahn
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·G SchiavoJ E Rothman
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·G SchiavoT H Söllner
Feb 28, 1998·Pflügers Archiv : European journal of physiology·S W SchneiderH Oberleithner
Jun 5, 1998·The Journal of Biological Chemistry·E R Chapman, A F Davis
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jihong BaiEdwin R Chapman
May 16, 2002·The Journal of Biological Chemistry·David E SaslowskyJ Michael Edwardson
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Edwin R Chapman
Sep 18, 2002·The Journal of Membrane Biology·N A GeisseJ M Edwardson
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yi WuSen-Fang Sui
Mar 1, 2003·Biophysical Journal·Jared C LawrenceRobert M Henderson
Jul 16, 2003·The Journal of Cell Biology·Ward C TuckerEdwin R Chapman
Mar 27, 2004·Science·Ward C TuckerEdwin R Chapman
Jun 26, 2004·Annual Review of Neuroscience·Thomas C Sudhof
Jul 9, 2004·Biophysical Journal·Travis HughesRobert C Davis
Dec 29, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Ralf P RichterAlain R Brisson
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Almut MeckeBradford G Orr
Mar 28, 2006·Nature Structural & Molecular Biology·Akhil BhallaEdwin R Chapman
Apr 6, 2006·The Journal of Biological Chemistry·LiYi LiChristian Rosenmund
Sep 26, 2006·Biophysical Journal·Andrea L SladeChristopher M Yip
Mar 21, 2007·Biophysical Journal·Gabriel Weinreb, Barry R Lentz
May 5, 2007·Science·Sascha MartensHarvey T McMahon

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Citations

Sep 13, 2012·Analytical and Bioanalytical Chemistry·Sandrine MorandatKarim El Kirat
Jun 7, 2011·Nature Structural & Molecular Biology·Enfu HuiEdwin R Chapman
May 26, 2011·The Journal of Biological Chemistry·Alex L LaiDavid S Cafiso
Jul 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Reut FriedrichUri Ashery
Jun 19, 2012·The EMBO Journal·Jörg MalsamThomas H Söllner
Oct 21, 2010·Biophysical Journal·Hirohide TakahashiJ Michael Edwardson
Nov 20, 2016·Annual Review of Physiology·Jarred M Whitlock, H Criss Hartzell
Aug 28, 2015·Nature Communications·Yuki SuzukiHiroshi Sugiyama
Oct 18, 2012·Physiological Reviews·Haruo KasaiHiroshi Tokumaru
Jun 10, 2010·Biochemistry·Swetha Suresh, J Michael Edwardson

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