Synaptotagmin VII restricts fusion pore expansion during lysosomal exocytosis

PLoS Biology
Jyoti K JaiswalSanford M Simon

Abstract

Synaptotagmin is considered a calcium-dependent trigger for regulated exocytosis. We examined the role of synaptotagmin VII (Syt VII) in the calcium-dependent exocytosis of individual lysosomes in wild-type (WT) and Syt VII knockout (KO) mouse embryonic fibroblasts (MEFs) using total internal reflection fluorescence microscopy. In WT MEFs, most lysosomes only partially released their contents, their membrane proteins did not diffuse into the plasma membrane, and inner diameters of their fusion pores were smaller than 30 nm. In Syt VII KO MEFs, not only was lysosomal exocytosis triggered by calcium, but all of these restrictions on fusion were also removed. These observations indicate that Syt VII does not function as the calcium-dependent trigger for lysosomal exocytosis. Instead, it restricts the kinetics and extent of calcium-dependent lysosomal fusion.

References

Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·K C MartinB K Kaang
Apr 1, 1995·Trends in Neurosciences·J T Littleton, H J Bellen
Mar 1, 1994·The Journal of Experimental Medicine·I TardieuxN W Andrews
Dec 15, 1996·Experientia·T H Söllner, J E Rothman
Apr 7, 1997·The Journal of Cell Biology·A RodríguezN W Andrews
Oct 1, 1996·Cell Structure and Function·T H Söllner, J E Rothman
Mar 26, 1999·Cell Calcium·R D Burgoyne, A Morgan
May 20, 1999·The Journal of Experimental Medicine·D BaramR Sagi-Eisenberg
Mar 22, 2000·The Journal of Cell Biology·I MartinezN W Andrews
Apr 4, 2000·The Journal of Cell Biology·J SchmoranzerS M Simon
May 9, 2001·The Journal of Experimental Medicine·E V CalerN W Andrews
Sep 25, 2001·Journal of Biosciences·J K Jaiswal
Jun 6, 2002·The Biochemical Journal·Ward C Tucker, Edwin R Chapman
Jun 20, 2002·The Biochemical Journal·Mitsunori FukudaKatsuhiko Mikoshiba
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Edwin R Chapman
Jul 18, 2002·The Journal of Cell Biology·Lara K MahalThomas H Söllner
Dec 7, 2002·Neuron·Motojiro Yoshihara, J Troy Littleton
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Phillip HolroydReinhard Jahn
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Justin W TaraskaWolfhard Almers
Jan 25, 2003·Nature Cell Biology·Geri KreitzerEnrique Rodriguez-Boulan
Apr 11, 2003·Molecular Biology of the Cell·Jan Schmoranzer, Sanford M Simon
Jun 6, 2003·Nature·Sunil P Gandhi, Charles F Stevens
Aug 20, 2003·The Journal of Cell Biology·Sabyasachi ChakrabartiNorma W Andrews
Dec 14, 2006·Journal of Cell Science·Elisabetta ZuccatoGillian M Griffiths

❮ Previous
Next ❯

Citations

Jun 20, 2014·Molecular Biology of the Cell·Tejeshwar C RaoArun Anantharam
Jan 20, 2007·Nature Chemical Biology·Jyoti K Jaiswal, Sanford M Simon
Feb 24, 2006·Molecular Biology of the Cell·Scott NolanEric Grote
Jan 19, 2007·Molecular Biology of the Cell·Tamar GattegnoBenjamin Podbilewicz
Mar 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jean-Sébastien SchonnJakob B Sørensen
Mar 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anton MaximovThomas C Südhof
Jun 20, 2008·The Journal of General Physiology·Alp YaradanakulDonald W Hilgemann
Jul 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Reut FriedrichUri Ashery
Dec 3, 2010·PloS One·Fannie W ChenYiannis A Ioannou
Nov 10, 2011·Cold Spring Harbor Perspectives in Biology·Thomas C Südhof
Jul 10, 2007·Nature Cell Biology·Zhijun ZhangShumin Duan
Apr 7, 2005·Molecular and Biochemical Parasitology·Sabyasachi ChakrabartiNorma W Andrews
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Paul L McNeil, Tom Kirchhausen
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Meyer B Jackson, Edwin R Chapman
Jun 1, 2006·The Journal of Cell Biology·Sascha NydeggerMarkus Thali
Jan 23, 2016·Biomedical Microdevices·Seonhee ParkMark Prausnitz
Jan 28, 2009·The Journal of Physiology·Natalia GustavssonWeiping Han
Jun 22, 2010·Current Opinion in Cell Biology·Zhiping P Pang, Thomas C Südhof
Dec 19, 2006·Current Opinion in Cell Biology·William M BementAndrew G Clark
Oct 21, 2015·Communicative & Integrative Biology·Kathrin Neuland, Manfred Frick
Feb 10, 2007·Biology of the Cell·Véronique Proux-GillardeauxThierry Galli
Mar 15, 2006·Annals of the New York Academy of Sciences·Richard A Steinhardt
Jun 1, 2012·Cellular Microbiology·Claire E Butler, Kevin M Tyler
Mar 17, 2016·ACS Applied Materials & Interfaces·Zi-Yi YuChris Abell
Oct 10, 2015·Cell Death and Differentiation·S C SreetamaJ K Jaiswal
Jul 1, 2006·Biochemical and Biophysical Research Communications·Xiong XiongTao Xu
Sep 20, 2005·Trends in Cell Biology·Norma W Andrews, Sabyasachi Chakrabarti
Sep 17, 2011·Bioarchitecture·Maria Teresa Abreu-BlancoSusan M Parkhurst
Jan 3, 2009·Methods in Cell Biology·Daniel Axelrod
Mar 29, 2014·Journal of Lipid Research·Mohammad Ali Samie, Haoxing Xu
Mar 30, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xavier MicheletMichael B Brenner
Dec 24, 2014·Nature Communications·Luana L SchefferJyoti K Jaiswal
Dec 8, 2016·MBio·Asuka Hirai-YukiStanley M Lemon
Nov 13, 2004·The Journal of Biological Chemistry·Sheldon S ShenRichard A Steinhardt
Jul 20, 2007·Nature Reviews. Molecular Cell Biology·J Paul LuzioNicholas A Bright
Sep 3, 2005·EMBO Reports·Norma W Andrews
Sep 4, 2015·Physiological Reviews·Sandra T Cooper, Paul L McNeil
Oct 5, 2007·American Journal of Physiology. Cell Physiology·William H RodenAmy B Harkins

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Methods Mentioned

BETA
fluorescence microscopy
transfection

Software Mentioned

MetaMorph

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