Synbiotic-driven improvement of metabolic disturbances is associated with changes in the gut microbiome in diet-induced obese mice

Molecular Metabolism
Xinxin KePeter C K Cheung

Abstract

The gut microbiota is an important influencing factor of metabolic health. Although dietary interventions with probiotics, prebiotics, and synbiotics can be effective means to regulate obesity and associated comorbidities, the underlying shifts in gut microbial communities, especially at the functional level, have not been characterized in great details. In this study, we sought to investigate the effects of synbiotics on the regulation of gut microbiota and the alleviation of high-fat diet (HFD)-induced metabolic disorders in mice. Specific pathogen-free (SPF) male C57BL/6J mice were fed diets with either 10% (normal diet, ND) or 60% (high-fat diet, HFD) of total calories from fat (lard). Dietary interventions in the HFD-fed mice included (i) probiotic (Bifidobacterium animalis subsp. lactis and Lactobacillus paracasei subsp. paracasei DSM 46331), (ii) prebiotic (oat β-glucan), and (iii) synbiotic (a mixture of i and ii) treatments for 12 weeks. Besides detailed characterization of host metabolic parameters, a multi-omics approach was used to systematically profile the microbial signatures at both the phylogenetic and functional levels using 16S rRNA gene sequencing, metaproteomics and targeted metabolomics analysis. The synbi...Continue Reading

Citations

Aug 31, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Adilah F AhmadNatalie C Ward
Jun 11, 2020·Critical Reviews in Food Science and Nutrition·Jagrani MinjRakesh Kumar Sharma
Sep 23, 2020·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Weigang GanJuan Meng
Nov 20, 2019·Biological Trace Element Research·Rong FuZilong Sun
Mar 24, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Qingqing LvDifei Wang
Jul 4, 2019·Physiological Reports·Praveen K DhandapaniHoward T Jacobs
Jan 1, 2019·Current Pharmacology Reports·Rachel GolonkaMatam Vijay-Kumar
Nov 13, 2020·Expert Opinion on Therapeutic Targets·Mohammad BadranDavid Gozal
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Kaicheng WenYue Zhu
Jan 27, 2021·Metabolism: Clinical and Experimental·Ge YangXin Jiang
Feb 11, 2021·International Journal of Molecular Sciences·Brandi MillerHariom Yadav
Aug 18, 2021·Food Research International·Chao TangYingjian Lu
Jan 2, 2022·Molecular Metabolism·Serena BoscainiJohn F Cryan

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJEB26534
PXD009564

Methods Mentioned

BETA
ELISA
Illumina sequencing

Software Mentioned

SPSS
Cytoscape
R
Image J
vegan
EzTaxon

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.