Synchronization of neuronal activity promotes survival of individual rat neocortical neurons in early development

The European Journal of Neuroscience
Thomas VoigtA Dolabela de Lima

Abstract

Neural activity is thought to play a significant role during the development of the cerebral cortex. In this study, we examined the effects of global activity block or enhancement and the effects of patterned firing on the ability of cultured rat neocortical neurons to survive during the second week in vitro, beyond the beginning of synaptogenesis. Blockade of neuronal activity by adding tetrodotoxin (TTX) and increasing magnesium concentration in the medium strongly reduced the survival of cortical cells. Increasing neuronal activity by raising the external potassium concentration significantly improved the survival of cortical neurons. We postulated that in a developing neuronal network the survival of nerve cells is regulated by synaptically mediated events that involve changes in the intracellular calcium concentration. To examine this question further, we monitored the activity of the developing network by optically recording the intracellular calcium signals of many neurons simultaneously. These recordings show that in low magnesium neocortical neurons express synchronized oscillation of their intracellular calcium concentration. The ability of a network to synchronize the changes in intracellular calcium of multiple cell...Continue Reading

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Citations

Jun 2, 1997·The Journal of Comparative Neurology·A D de LimaT Voigt
Dec 31, 2002·Brain Research. Brain Research Reviews·Kwang-Hyuk LeeEvian Gordon
Dec 18, 2001·Brain Research. Developmental Brain Research·I E KudryashovN V Gulyaeva
Oct 12, 2007·Physical Biology·Radhika MadhavanSteve M Potter
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Jan 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A WagenaarSteve M Potter
Sep 21, 2010·Biomaterials·Midori Kato-NegishiShoji Takeuchi
Dec 5, 2014·PLoS Computational Biology·Julijana GjorgjievaAdrienne L Fairhall
Jul 22, 2016·PloS One·Jeffrey H ZimeringZhongcong Xie
Jun 9, 2004·The European Journal of Neuroscience·Ana Dolabela de LimaThomas Voigt
Jan 24, 2007·The European Journal of Neuroscience·Ana D de LimaThomas Voigt
Apr 24, 2008·Journal of Biomedical Materials Research. Part a·Varadraj N VernekarMichelle C LaPlaca
Dec 17, 2008·Developmental Neurobiology·Ana D de LimaThomas Voigt
Mar 18, 2020·Advanced Healthcare Materials·Dieter JanzenCarmen Villmann
Jul 10, 2019·Annual Review of Cell and Developmental Biology·Fong Kuan Wong, Oscar Marín

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