Jul 12, 2017

Synchronized DNA cycling across a bacterial population

Nature Genetics
Leo BaumgartJeff Hasty

Abstract

A defining goal of synthetic biology is to engineer cells to coordinate tasks that often require precise temporal modulation of gene expression. Although a variety of relatively small gene circuits have been constructed and characterized, their logical combination into larger networks remains a central challenge. This is due primarily to the lack of compatible and orthogonal elements for predictable dynamic control of gene expression. As an alternative approach to promoter-level regulation, we explored the use of DNA copy number as a circuit control element. We engineered colony-wide DNA cycling in Escherichia coli in the form of plasmid copy number oscillations via a modular design that can be readily adapted for use with other gene circuitry. Copy number modulation is a generalizable principle that adds a layer of control to synthetic gene circuits, allowing dynamic regulation of circuit elements without requiring specially engineered promoters.

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  • Citations2

Citations

Mentioned in this Paper

Gene Expression Regulation, Bacterial
Alkalescens-Dispar Group
DNA Copy Number Changes
Regulation of Biological Process
Promoter
Neuronal Circuitry
Gene Expression
Bacterial Artificial Chromosomes
Plasmids
Escherichia coli

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