Abstract
Periodic secretion of GnRH from the hypothalamus is the driving force for the release of gonadotropic hormones from the pituitary, but the roles of individual neurons in the context of this pulse generator are not known. In this study we used FM1-43 to monitor the membrane turnover associated with exocytosis in single GT1-7 neurons and found an intrinsic secretory pulsatility (frequency, 1.4 +/- 0.1/h; pulse duration, 17.3 +/- 0.6 min) that, during time in culture, became progressively synchronized among neighboring cells. Voltage-gated calcium channels and gap junctional communication each played a major role in synchronized pulsatility. An L-type calcium channel inhibitor, nimodipine, abolished synchronized pulsatility. In addition, functional gap junction communication among adjacent cells was detected, but only under conditions where pulsatile synchronization was also observed, and the gap junction inhibitor octanol abolished both without affecting pulse frequency or duration. Our results, therefore, provide strong evidence that the GnRH pulse generator in GT1-7 cells arises from a single cell oscillator mechanism that is synchronized through network signaling involving voltage-gated calcium channels and gap junctions.
References
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Z KrsmanovićK J Catt
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Martínez de la EscaleraR I Weiner
Jul 1, 1990·Neuron·P L MellonR I Weiner
Aug 29, 1986·Science·R D Veenstra, R L DeHaan
Aug 1, 1988·Endocrinology·C Masotto, A Negro-Vilar
Oct 1, 1993·The Journal of Membrane Biology·M M Bosma
Feb 1, 1995·Cellular and Molecular Neurobiology·E Terasawa
Feb 1, 1995·Cellular and Molecular Neurobiology·W C Wetsel
Jan 1, 1995·Journal of Neurophysiology·A C Charles, T G Hales
Mar 1, 1995·Neuroendocrinology·G Martínez de la EscaleraR I Weiner
Feb 1, 1993·Bioscience Reports·S J StaffordJ G Schofield
Nov 1, 1993·Neuroendocrinology·D F MatesicB V Madhukar
Mar 1, 1994·Neuroendocrinology·T G HalesA C Charles
Nov 5, 1993·Cell·T SöllnerJ E Rothman
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·P P MehtaM C Wilson
Oct 1, 1996·Neuroendocrinology·D F MatesicJ A Germak
Mar 21, 1998·Neuroendocrinology·S Hosny, L Jennes
Jul 24, 1998·Biochimica Et Biophysica Acta·Y KamC O Joe
Dec 9, 1998·General and Comparative Endocrinology·E Terasawa
Mar 6, 1999·Endocrinology·L Z KrsmanovicK J Catt
Mar 30, 1999·Brain Research·T S KingM A Javors
Apr 15, 1999·Annual Review of Neuroscience·A J CochillaW J Betz
May 13, 1999·Nature Neuroscience·J K AnglesonW J Betz
Jul 13, 1999·Journal of Neurophysiology·J L Costantin, A C Charles
Aug 31, 1999·Biophysical Journal·T Höfer
Oct 26, 1999·Neuroendocrinology·L HuP C Goldsmith
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E A VitalisR I Weiner
Jun 1, 2000·Endocrinology·L NúñezL S Frawley
Aug 23, 2000·The Journal of Cell Biology·A J CochillaW J Betz
Oct 3, 2000·Endocrinology·K J SuterS M Moenter
Citations
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Han Kyoung ChoeKyungjin Kim
Jun 18, 2005·Endocrinology·Nurul KabirDipak K Sarkar
Mar 20, 2010·Endocrine Reviews·Catherine A Christian, Suzanne M Moenter
Jun 17, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhiguo Chu, Suzanne M Moenter
Feb 25, 2014·Frontiers in Neuroendocrinology·David J Lyons, Christian Broberger
Jan 31, 2004·Neurobiology of Disease·Malin K SandbergKrister Kristensson
Nov 1, 2002·General and Comparative Endocrinology·Gilles M LeclercFredric R Boockfor
Jul 3, 2010·Frontiers in Neuroendocrinology·Christine L JasoniAllan E Herbison
Oct 13, 2009·Cell Calcium·Hanaa HoddahEmilio Carbone
Dec 17, 2008·Neurochemistry International·Patricia VarjuZsolt Liposits
Jan 24, 2007·Molecular and Cellular Endocrinology·Gilles M Leclerc, Fredric R Boockfor
Jul 8, 2008·Journal of Neuroendocrinology·S B Seminara, W F Crowley
Mar 30, 2011·Journal of Neuroendocrinology·S Constantin
Jun 15, 2013·Journal of Neuroendocrinology·S L PetersenJ A Lopez
May 25, 2005·Brain Research·Maria Gulinello, Anne M Etgen
Sep 8, 2004·Journal of the Society for Gynecologic Investigation·Eileen C ChenThomas S King
Aug 29, 2002·Molecular Endocrinology·Rafael Vazquez-MartinezFredric R Boockfor
Nov 13, 2015·Endocrinology·Caroline Pinet-CharvetAnne Duittoz
Nov 20, 2001·Endocrinology·R Vazquez-MartinezF R Boockfor
Sep 28, 2006·American Journal of Physiology. Endocrinology and Metabolism·Naibedya ChattopadhyayEdward M Brown
Oct 21, 2011·Physiological Reviews·Domenico BoscoPaolo Meda
Sep 5, 2014·Journal of Neurophysiology·Zahra DargaeiNeil S Magoski
Nov 1, 2003·Endocrinology·Anne GrangerJean-Noël Laverrière
Oct 16, 2004·Endocrinology·T J WuJames L Roberts
Apr 12, 2008·Endocrinology·Kosuke Haneda, Yoshitaka Oka
Mar 31, 2011·Endocrinology·Rebecca E CampbellAllan E Herbison
May 22, 2002·Endocrinology·Craig S NunemakerSuzanne M Moenter
Aug 10, 2017·Frontiers in Endocrinology·Stephanie Constantin
Jan 8, 2004·Experimental Biology and Medicine·Michael J WollerDaryle Waechter-Brulla
Dec 13, 2005·Molecular and Cellular Endocrinology·Gilles M Leclerc, Fredric R Boockfor