Synchronized mechanical oscillations at the cell-matrix interface in the formation of tensile tissue

Proceedings of the National Academy of Sciences of the United States of America
David F HolmesKarl E Kadler

Abstract

The formation of uniaxial fibrous tissues with defined viscoelastic properties implies the existence of an orchestrated mechanical interaction between the cytoskeleton and the extracellular matrix. This study addresses the nature of this interaction. The hypothesis is that this mechanical interplay underpins the mechanical development of the tissue. In embryonic tendon tissue, an early event in the development of a mechanically robust tissue is the interaction of the pointed tips of extracellular collagen fibrils with the fibroblast plasma membrane to form stable interface structures (fibripositors). Here, we used a fibroblast-generated tissue that is structurally and mechanically matched to embryonic tendon to demonstrate homeostasis of cell-derived and external strain-derived tension over repeated cycles of strain and relaxation. A cell-derived oscillatory tension component is evident in this matrix construct. This oscillatory tension involves synchronization of individual cell forces across the construct and is induced in each strain cycle by transient relaxation and transient tensioning of the tissue. The cell-derived tension along with the oscillatory component is absent in the presence of blebbistatin, which disrupts acti...Continue Reading

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Citations

Nov 7, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andreas HerchenhanPernilla Eliasson
Jan 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christopher L GilchristFarshid Guilak
Apr 16, 2021·Journal of the Mechanical Behavior of Biomedical Materials·A GiannopoulosS P Magnusson
Jun 24, 2021·BioMed Research International·Ryan A Behmer HansenSamuel E Senyo
Sep 11, 2021·ACS Biomaterials Science & Engineering·Cassandra L MartinLeila F Deravi
Jul 7, 2021·Nature Communications·Joseph d'AlessandroBenoît Ladoux

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