Synchronized spontaneous Ca2+ transients in acute anterior pituitary slices.

The Journal of Biological Chemistry
N C GuérineauP Mollard

Abstract

We investigated the organization of spontaneous rises in cytosolic free Ca2+ concentration ([Ca2+]i) due to electrical activity in acute pituitary slices. Real time confocal imaging revealed that 73% of the cells generated fast peaking spontaneous [Ca2+]i transients. Strikingly, groups of apposing cells enhanced their [Ca2+]i in synchrony with a speed of coactivation >1,000 microm/s. Single-cell injection of Neurobiotin or Lucifer yellow labeled clusters of cells, which corresponded to coactive cells. Halothane, a gap junction blocker, markedly reduced the spread of tracers. Coupling between excitable cells was mainly homologous in nature, with a prevalence of growth hormone-containing cells. We conclude that spontaneously active endocrine cells are either single units or arranged in synchronized gap junction-coupled assemblies scattered throughout the anterior pituitary gland. Synchrony between spontaneously excitable cells may help shape the patterns of basal secretion.

References

Nov 11, 1975·The Journal of Cell Biology·W H FletcherJ W Everett
Jul 1, 1992·Pflügers Archiv : European journal of physiology·R Schneggenburger, A Konnerth
Jun 18, 1990·Molecular and Cellular Endocrinology·W AllaertsC Denef
Sep 1, 1989·Pflügers Archiv : European journal of physiology·F A EdwardsT Takahashi
Aug 1, 1989·The Anatomical Record·T Soji, D C Herbert
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C SáezM V Bennett
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C SaezM V Bennett
Jan 1, 1971·Neuroendocrinology·D H YorkJ Kraicer
Jul 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A BerodJ F Pujol
Jul 31, 1980·Nature·M F JohnstonF Ramón
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1995·Annual Review of Neuroscience·W Singer, C M Gray
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·P MollardM C Nowycky
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z P ChenS L Lightman
Feb 7, 1997·Science·E A Newman, K R Zahs
May 16, 1997·Journal of Neuroscience Methods·N C GuérineauB H Gähwiler

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Citations

Feb 5, 2003·Biology of the Cell·Véronique Serre-BeinierPaolo Meda
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·S S Stojilkovic
Aug 7, 2002·Trends in Endocrinology and Metabolism : TEM·Teddy FauquierPatrice Mollard
Jan 5, 2012·Nature Communications·David J HodsonPatrice Mollard
Dec 21, 2013·Nature Communications·Michel G DesarménienNathalie C Guérineau
Jul 25, 2000·Acta Physiologica Scandinavica·J Røttingen, J G Iversen
Sep 1, 1999·Clinical Endocrinology·S Chappel
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chrystel LafontPatrice Mollard
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Claudia Sanchez-CardenasPatrice Mollard
May 12, 2009·The Journal of Biological Chemistry·Barthélémy DioufDominique Joubert
Mar 2, 2006·Molecular and Cellular Biology·Alejandra CollazosDominique Joubert
Apr 28, 2004·Journal of Biomaterials Science. Polymer Edition·Nicholas A Peppas, William Leobandung
Apr 7, 2011·Endocrinology·Shuo LiStanko S Stojilkovic
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·T FauquierP Mollard
Nov 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xavier BonnefontPatrice Mollard
May 12, 2006·Médecine sciences : M/S·Xavier BonnefontPatrice Mollard
Aug 3, 2010·Journal of Neuroendocrinology·D J HodsonP Mollard
Jan 15, 2010·Journal of Neuroendocrinology·X Bonnefont
Dec 20, 2005·Biochimica Et Biophysica Acta·Laetitia MichonPaolo Meda
Oct 18, 2011·Biochimica Et Biophysica Acta·Ilaria PotolicchioPaolo Meda
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·David J HodsonNathalie C Guérineau
Sep 18, 2012·Frontiers in Neuroendocrinology·P R Le TissierP Mollard
Sep 3, 2016·Frontiers in Molecular Neuroscience·Christina GöngrichHannah Monyer
Jun 28, 2017·Molecular and Cellular Endocrinology·Patrick A FletcherStanko S Stojilkovic
Mar 4, 2000·Endocrinology·X BonnefontP Mollard
Oct 21, 2011·Physiological Reviews·Domenico BoscoPaolo Meda
Dec 29, 1998·The Journal of Biological Chemistry·M ChansonH J Jongsma
Oct 27, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B Mary LewisJack Ham
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y AshinoJ Bhattacharya
Feb 24, 2001·Annals of the New York Academy of Sciences·F JamenP Brabet
Feb 24, 2001·Annals of the New York Academy of Sciences·P KunzelmannP Mollard
Feb 16, 2020·Scientific Reports·Tamara SerdinšekJurij Dolenšek
Feb 16, 2021·Frontiers in Endocrinology·Yorgui Santiago-AndresTatiana Fiordelisio
Jun 12, 1999·Biochemical and Biophysical Research Communications·H TakemuraA Segawa

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