Synchronous fluorescence spectroscopic characterization of DMBA-TPA-induced squamous cell carcinoma in mice

Journal of Biomedical Optics
Parmeswaran DiagaradjaneBahman Anvari

Abstract

While initially confined to the epidermis, squamous cell carcinoma can eventually penetrate into the underlying tissue if not diagnosed early and treated. The noninvasive early detection of the carcinoma is important to achieve a complete treatment of the disease. Of the various non-invasive optical techniques, the synchronous fluorescence (SF) technique is considered to provide a simplified spectral profile with more sharp spectral signatures of the endogenous fluorophores in complex systems. The potential use of the SF technique in the characterization of the sequential tissue transformation in 7,12-dimethylbenz(a)anthracene-12-O-tetradecanoylphorbol-13-acetate (DMBA-TPA)-induced mouse skin tumor model in conjunction with simple statistical analysis is explored. The SF spectra show distinct differences during the earlier weeks of the tumor-induction period. Intensity ratio variables are calculated and used in three discriminant analyses. All the discriminant analyses show better classification results with accuracy greater than 80%. From the observed differences in the spectral characteristics and the ratio variables that resulted in better classification between groups, it is concluded that tryptophan, collagen, and NADH are...Continue Reading

References

Jan 1, 1992·Lasers in Surgery and Medicine·K T SchomackerT F Deutsch
Jul 1, 1972·The American Journal of Physiology·B ChanceA G Kovach
Jan 1, 1966·Acta Medica Scandinavica. Supplementum·C Rimington
Jan 1, 1982·Journal of Cellular Biochemistry·T J SlagaJ Reiners
Nov 1, 1995·Archives of Otolaryngology--head & Neck Surgery·V R KolliS P Schantz
Jan 1, 1994·Advances in Experimental Medicine and Biology·S NiokaB Chance
Jan 1, 1993·Lasers in Surgery and Medicine·A MahadevanR R Richards-Kortum
Sep 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·M MoniciG Bottiroli
Jan 1, 1996·Annual Review of Physical Chemistry·R Richards-Kortum, E Sevick-Muraca
Jan 1, 1997·Head & Neck·D R IngramsM S Feld
Oct 24, 1998·Gastrointestinal Endoscopy·M A MycekN S Nishioka
Nov 24, 1998·Photochemistry and Photobiology·G A WagnièresB C Wilson
Jun 1, 1996·Physical Review C: Nuclear Physics·P V GreenI J Thompson
Sep 24, 1999·Lasers in Surgery and Medicine·A AgrawalR Richards-Kortum
Jul 27, 2000·Photochemistry and Photobiology·D L HeintzelmanR R Richards-Kortum
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·N Ramanujam
Feb 14, 2003·International Journal of Cancer. Journal International Du Cancer·Wei ZhengMalini Olivo
Jul 23, 2003·Photochemistry and Photobiology·Jane Sandby-MøllerHans Christian Wulf
Aug 30, 2003·Photochemistry and Photobiology·S MadhuriS Ganesan
Nov 19, 2003·IEEE Transactions on Bio-medical Engineering·Gregory M PalmerNirmala Ramanujam
Jan 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·B K ManjunathV B Kartha
Mar 1, 1978·Analytical Chemistry·T Vo-Dinh

❮ Previous
Next ❯

Citations

Oct 23, 2009·Photochemistry and Photobiology·Jeyasingh EbenezarSingaravelu Ganesan
Sep 26, 2015·Sensors·Xiaofeng ZhangTuan Vo-Dinh
Apr 26, 2012·Toxicology and Applied Pharmacology·Poulomi Bhattacharya, Aileen F Keating
Jul 3, 2015·Journal of Photochemistry and Photobiology. B, Biology·Seema DeviAsima Pradhan
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.