Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies.

Nature Protocols
Jonah B Sacha, David I Watkins

Abstract

The development of an HIV vaccine will require a more precise understanding of the immunological and virological underpinnings of HIV infection. Magnetofection, the process of magnetizing HIV by coupling it to ferrous nanoparticles and coordinating infection using a magnetic field, synchronizes the viral replication cycle at attachment while recapitulating the events of natural infection. Although spinoculation also concentrates virus onto target cells to increase infection, it does not synchronize infection. The synchronization of HIV infection in vitro facilitates the study of events in the viral replication cycle and the antiviral immune response on timelines previously impossible. Furthermore, by infecting a high percentage of cells in a short time frame, magnetofection increases the throughput of in vitro assays. Once a virus stock is generated, magnetofection of target cells is rapid, requiring only 1-2 h. Here we present a detailed protocol for this assay and review its applications for studying the immune response to HIV.

References

Dec 15, 2004·Journal of Virology·Hillel HaimAmos Panet
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonah B SachaDavid I Watkins
Oct 20, 2007·Nature Protocols·Olga MykhaylykChristian Plank
Oct 24, 2007·The Journal of Experimental Medicine·Nicholas J ManessDavid I Watkins
Dec 15, 2007·Nature Protocols·Thomas BuerliChristian Fuhrer
Mar 8, 2008·Nature Protocols·Angélique B van 't WoutNeeltje A Kootstra
May 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jonah B SachaDavid I Watkins

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Citations

Dec 8, 2011·Pharmaceutical Research·Cédric SapetOlivier Zelphati
Sep 7, 2011·Advanced Drug Delivery Reviews·Christian PlankOlga Mykhaylyk
Dec 4, 2014·Cell Host & Microbe·Amy E BaxterQuentin J Sattentau
Sep 13, 2013·Nature·Scott G HansenLouis J Picker

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