Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores.

The Review of Scientific Instruments
Gautam V SoniAmit Meller

Abstract

We present a novel method for integrating two single-molecule measurement modalities, namely, total internal reflection microscopy and electrical detection of biomolecules using nanopores. Demonstrated here is the electrical measurement of nanopore based biosensing performed simultaneously and in-sync with optical detection of analytes. This method makes it possible, for the first time, to visualize DNA and DNA-protein complexes translocating through a nanopore with high temporal resolution (1000 frames/s) and good signal to background. This paper describes a detailed experimental design of custom optics and data acquisition hardware to achieve simultaneous high resolution electrical and optical measurements on labeled biomolecules as they traverse through a approximately 4 nm synthetic pore. In conclusion, we discuss new directions and measurements, which this technique opens up.

References

Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·A MellerD Branton
May 1, 2001·Physical Review Letters·A MellerD Branton
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A J StormC Dekker
Sep 15, 2005·Nano Letters·Daniel FologeaJiali Li
Apr 13, 2006·Nano Letters·Johan LagerqvistMassimiliano Di Ventra
Mar 7, 2007·Nature Methods·Breton HornblowerMark Akeson
Aug 28, 2007·Nano Letters·Guillaume A T ChansinJoshua B Edel
Sep 25, 2007·Clinical Chemistry·Gautam V Soni, Amit Meller
Dec 12, 2007·Nano Letters·Grigori SigalovAleksei Aksimentiev
Dec 22, 2007·Nanomedicine·Ken HealyAlan P Morrison
Apr 9, 2008·Applied Physics Letters·Daniel FologeaJiali Li
Jul 26, 2008·Nature Nanotechnology·Cees Dekker
Aug 19, 2008·Biophysical Journal·Meni WanunuAmit Meller
Oct 11, 2008·Nature Biotechnology·Daniel BrantonJeffery A Schloss
Jul 9, 2009·Sensors and Actuators. B, Chemical·Brian C GierhartScott D Collins
Jul 23, 2009·Chemical Society Reviews·Stefan Howorka, Zuzanna Siwy
Oct 3, 2012·Cold Spring Harbor Protocols·Chirlmin Joo, Taekjip Ha
Nov 24, 2012·Bioanalysis·Christopher J KemperMichael Koleto

❮ Previous
Next ❯

Citations

May 9, 2012·IET Nanobiotechnology·H Pradeep, G K Rajanikant
Jul 1, 2011·Journal of the Royal Society, Interface·Ulrich F Keyser
Apr 6, 2016·Mikrochimica Acta·Shuo LiuHolger Schmidt
Jun 5, 2012·Physics of Life Reviews·Meni Wanunu
Aug 30, 2011·Trends in Biotechnology·Stefan W KowalczykCees Dekker
Nov 25, 2014·Biophysical Journal·Spencer CarsonMeni Wanunu
Jul 24, 2012·Biochemical Society Transactions·Azadeh BahramiTim Albrecht
May 21, 2013·Scientific Reports·Naoya YukimotoMasateru Taniguchi
May 20, 2015·The Analyst·Zachary D HarmsStephen C Jacobson
Dec 24, 2014·Chemical Communications : Chem Comm·Shuo LiuHolger Schmidt
Feb 17, 2015·The Analyst·Tal Gilboa, Amit Meller
Oct 20, 2018·Small·Yuning ZhangWilliam B Dunbar
Feb 23, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Mariam AyubTim Albrecht
Oct 31, 2012·Electrophoresis·Evrim AtasAmit Meller
Sep 20, 2012·Chemical Society Reviews·Benjamin N MilesJoshua B Edel
Feb 3, 2018·The Review of Scientific Instruments·Zachary RoelenVincent Tabard-Cossa
Mar 27, 2020·Micromachines·Jiye FuZuhong Lu
Aug 11, 2017·Nanoscale·Jiwook ShimRashid Bashir
Mar 3, 2019·The Journal of Chemical Physics·Ilya SychugovJan Linnros
Dec 14, 2018·Nanotechnology·Kamyar Akbari RoshanWeihua Guan
May 20, 2014·Nanoscale·Furat SawaftaAdam R Hall
Jun 18, 2021·Lab on a Chip·Mahmudur RahmanHolger Schmidt
Sep 13, 2017·The Journal of Physical Chemistry. B·Yuta KatoToshiyuki Mitsui
Mar 18, 2020·Analytical Chemistry·Si-Min LuYi-Tao Long
Oct 28, 2014·Nano Letters·Francesca NicoliMagnus P Jonsson
Nov 12, 2019·Nano Letters·Michael GrafAleksandra Radenovic

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence resonance
chip
FRET

Software Mentioned

LABVIEW

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.