Synchrotron X-ray footprinting on tour

Journal of Synchrotron Radiation
Jen BohonMark R Chance

Abstract

Synchrotron footprinting is a valuable technique in structural biology for understanding macromolecular solution-state structure and dynamics of proteins and nucleic acids. Although an extremely powerful tool, there is currently only a single facility in the USA, the X28C beamline at the National Synchrotron Light Source (NSLS), dedicated to providing infrastructure, technology development and support for these studies. The high flux density of the focused white beam and variety of specialized exposure environments available at X28C enables footprinting of highly complex biological systems; however, it is likely that a significant fraction of interesting experiments could be performed at unspecialized facilities. In an effort to investigate the viability of a beamline-flexible footprinting program, a standard sample was taken on tour around the nation to be exposed at several US synchrotrons. This work describes how a relatively simple and transportable apparatus can allow beamlines at the NSLS, CHESS, APS and ALS to be used for synchrotron footprinting in a general user mode that can provide useful results.

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Citations

Mar 27, 2016·Methods : a Companion to Methods in Enzymology·Ryan M HulscherSarah A Woodson
Jun 28, 2014·Journal of Synchrotron Radiation·Sayan GuptaCorie Ralston
Sep 1, 2016·Journal of Synchrotron Radiation·Sayan GuptaCorie Y Ralston
Nov 30, 2018·Protein and Peptide Letters·Jen Bohon
Nov 30, 2018·Protein and Peptide Letters·Simon A MortonCorie Y Ralston
Jul 6, 2019·Journal of Synchrotron Radiation·Awuri AsuruJen Bohon
Nov 1, 2015·Biochemical Society Transactions·Vassiliy N BavroCorie Ralston
Jun 22, 2018·Current Protocols in Nucleic Acid Chemistry·Yumeng HaoSarah A Woodson
Sep 7, 2020·Biophysical Journal·Paige WheatleyShahid Khan
Feb 24, 2020·Journal of Molecular Biology·Mark R ChanceJanna Kiselar
Dec 4, 2019·Analytical Chemistry·Sayan GuptaCorie Y Ralston

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