Synchrotron X-ray imaging of pulmonary alveoli in respiration in live intact mice

Scientific Reports
Soeun ChangAkira Tsuda

Abstract

Despite nearly a half century of studies, it has not been fully understood how pulmonary alveoli, the elementary gas exchange units in mammalian lungs, inflate and deflate during respiration. Understanding alveolar dynamics is crucial for treating patients with pulmonary diseases. In-vivo, real-time visualization of the alveoli during respiration has been hampered by active lung movement. Previous studies have been therefore limited to alveoli at lung apices or subpleural alveoli under open thorax conditions. Here we report direct and real-time visualization of alveoli of live intact mice during respiration using tracking X-ray microscopy. Our studies, for the first time, determine the alveolar size of normal mice in respiration without positive end expiratory pressure as 58 ± 14 (mean ± s.d.) μm on average, accurately measured in the lung bases as well as the apices. Individual alveoli of normal lungs clearly show heterogeneous inflation from zero to ~25% (6.7 ± 4.7% (mean ± s.d.)) in size. The degree of inflation is higher in the lung bases (8.7 ± 4.3% (mean ± s.d.)) than in the apices (5.7 ± 3.2% (mean ± s.d.)). The fraction of the total tidal volume allocated for alveolar inflation is 34 ± 3.8% (mean ± s.e.m). This study co...Continue Reading

References

Nov 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·H BachofenE R Weibel
Feb 1, 1987·Journal of Applied Physiology·T A WilsonJ R Rodarte
May 1, 1987·Journal of Applied Physiology·H BachofenE R Weibel
Mar 1, 1974·Respiration Physiology·R ArdilaJ Hildebrandt
Oct 1, 1993·Journal of Applied Physiology·H MikiJ L Lehr
Sep 1, 1995·Journal of Applied Physiology·A TsudaJ P Butler
Mar 6, 1999·Journal of Applied Physiology·A TsudaJ P Butler
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
Jun 1, 2001·Critical Care Medicine·H J SchillerG F Nieman
Jun 13, 2001·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·E O GerscovichC McDonald
Feb 26, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·D M HydeH J G Gundersen
Mar 10, 2004·Physics in Medicine and Biology·Y HwuJe-Kyung Seong
Oct 7, 2004·Biophysical Journal·Y HwuG Margaritondo
Feb 18, 2005·Physics in Medicine and Biology·Sylvie MonfraixAnssi R A Sovijärvi
Mar 9, 2005·Critical Care Medicine·David CarneyGary Nieman
May 9, 2006·Respiratory Physiology & Neurobiology·A J Esbaugh, B L Tufts
Jul 14, 2007·American Journal of Respiratory and Critical Care Medicine·Scott E SinclairChristopher M Waters
Nov 23, 2007·The European Respiratory Journal·H FehrenbachJ R Nyengaard
Dec 31, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Juliane KnustJens R Nyengaard
Jul 23, 2009·Critical Care Medicine·Michael MertensWolfgang M Kuebler
Jul 10, 2010·Journal of Biomedical Optics·Sven MeissnerEdmund Koch
Nov 13, 2010·Journal of Applied Physiology·Annalisa J Swan, Merryn H Tawhai
Dec 15, 2010·Nature Methods·Mark R LooneyMatthew F Krummel
Aug 2, 2011·Journal of Theoretical Biology·Akira TsudaPeter E Hydon
Aug 30, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Esra Roan, Christopher M Waters
Apr 12, 2012·Journal of the Royal Society, Interface·Stephen DubskyAndreas Fouras
Jun 29, 2012·Scientific Reports·Jinkyung KimJung Ho Je
Jul 17, 2012·Journal of Applied Physiology·Gerald C Smaldone, Wayne Mitzner
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dragoş M VasilescuEric A Hoffman
Dec 19, 2012·Scientific Reports·Jinkyung KimIm Joo Rhyu
Feb 19, 2013·Scientific Reports·Soeun ChangJung Ho Je
Nov 23, 2013·Comprehensive Physiology·Akira TsudaJames P Butler
Nov 29, 2013·The New England Journal of Medicine·Arthur S Slutsky, V Marco Ranieri

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Citations

Aug 5, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Luosha XiaoShigeo Wada
Dec 14, 2016·Scientific Reports·Daniel H LarssonHans M Hertz
Apr 30, 2017·Journal of Synchrotron Radiation·P DemanN L Ford
Nov 6, 2018·Histochemistry and Cell Biology·Lars Knudsen, Matthias Ochs
Mar 14, 2020·Scientific Reports·Jiwon LeeOnseok Lee
Jul 23, 2020·Frontiers in Physiology·Bradford J SmithJason H T Bates
Mar 28, 2017·Scientific Reports·Kai SchererJulia Herzen
Jan 13, 2018·Biomaterials Science·James C H PoonThomas K Waddell
Feb 26, 2019·Frontiers in Immunology·Marco BlickensdorfMarc Thilo Figge
Jul 28, 2019·Intensive Care Medicine Experimental·Jana GruneWolfgang M Kuebler
Jun 20, 2020·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Kai RuppertRahim R Rizi
Jun 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jason M ColeZulfikar Najmudin
Oct 25, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Alec N Thomas, Mark A Borden

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Methods Mentioned

BETA
X-ray

Software Mentioned

TrXM

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