Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis.

Nature Neuroscience
Victor AnggonoPhillip J Robinson

Abstract

Dynamin I is dephosphorylated at Ser-774 and Ser-778 during synaptic vesicle endocytosis (SVE) in nerve terminals. Phosphorylation was proposed to regulate the assembly of an endocytic protein complex with amphiphysin or endophilin. Instead, we found it recruits syndapin I for SVE and does not control amphiphysin or endophilin binding in rat synaptosomes. After depolarization, syndapin showed a calcineurin-mediated interaction with dynamin. A peptide mimicking the phosphorylation sites disrupted the dynamin-syndapin complex, not the dynamin-endophilin complex, arrested SVE and produced glutamate release fatigue after repetitive stimulation. Pseudophosphorylation of Ser-774 or Ser-778 inhibited syndapin binding without affecting amphiphysin recruitment. Site mutagenesis to alanine arrested SVE in cultured neurons. The effects of the sites were additive for syndapin I binding and SVE. Thus syndapin I is a central component of the endocytic protein complex for SVE via stimulus-dependent recruitment to dynamin I and has a key role in synaptic transmission.

References

Apr 30, 1986·Brain Research·P R DunkleyJ A Rostas
Aug 1, 1994·Trends in Neurosciences·P J RobinsonT C Südhof
Jul 29, 1994·Biochemical and Biophysical Research Communications·H HosoyaK Yazaki
Aug 1, 1993·The Journal of Cell Biology·A M van der BliekS L Schmid
Aug 1, 1993·The Journal of Cell Biology·J S HerskovitsR B Vallee
Oct 1, 1995·Endocrine Reviews·J P Liu, P J Robinson
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R UrrutiaM A McNiven
Mar 28, 1997·The Journal of Biological Chemistry·E de HeuvelP S McPherson
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·N RingstadP De Camilli
Mar 28, 1998·The Journal of Biological Chemistry·R ShuangE L Stuenkel
Sep 3, 1998·Molecular Biology of the Cell·H CaoM A McNiven
Sep 3, 1999·The Journal of Biological Chemistry·M M LaiS H Snyder
Apr 15, 2000·The Journal of Biological Chemistry·K A PowellP J Robinson
Aug 10, 2000·The Journal of Biological Chemistry·M M LaiS H Snyder
Sep 15, 2000·Journal of Neurochemistry·M A Cousin, P J Robinson
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·R Dhavan, L H Tsai
Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Oct 24, 2001·Trends in Neurosciences·M A Cousin, P J Robinson
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eunkyung Lee, Pietro De Camilli
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·James D OrthMark A McNiven
Mar 6, 2002·The Journal of Biological Chemistry·Mo-Chou Chen-HwangYu-Wen Hwang

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Citations

Nov 10, 2010·Cellular and Molecular Neurobiology·Shyue-An ChanCorey Smith
Nov 22, 2012·Journal of Medicinal Chemistry·Christopher P GordonAdam McCluskey
Jul 7, 2012·Nature Protocols·James A DanielPhillip J Robinson
Jan 12, 2012·Nature Reviews. Molecular Cell Biology·Shawn M Ferguson, Pietro De Camilli
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qi WangHolger Sondermann
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Arndt PechsteinVolker Haucke
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yijian RaoVolker Haucke
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ya-Wen LiuSandra L Schmid
Feb 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Annie QuanPhillip J Robinson
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xuelin LouPietro De Camilli
Nov 12, 2009·The Journal of Biological Chemistry·Nina KahlfeldtVolker Haucke
Jul 7, 2011·The Journal of Biological Chemistry·Jing XuePhillip J Robinson
Feb 16, 2012·The Journal of Biological Chemistry·Luigi RacioppiAnthony R Means
Dec 29, 2010·The Journal of General Physiology·Vincenzo LaricciaDonald W Hilgemann
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Jeremy Dittman, Timothy A Ryan
Apr 12, 2012·American Journal of Physiology. Cell Physiology·Susanne G Straub, Geoffrey W G Sharp
May 16, 2009·Circulation Research·Sarah M SchumacherJeffrey R Martens
Sep 18, 2009·Journal of Cell Science·Jürgen A W Heymann, Jenny E Hinshaw
Jan 19, 2012·PLoS Computational Biology·Eyal AkivaHanah Margalit
Jul 7, 2011·PloS One·Tsutomu OkaHitoshi Okazawa
Dec 14, 2012·PloS One·Shih Lin GohHolger Sondermann
Jun 27, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·J BalajiTimothy A Ryan
Jul 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Prattana SamasilpCorey Smith
Sep 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tao SunLing-Gang Wu
Jun 19, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Emma L ClaytonMichael A Cousin
Jan 12, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gareth J O Evans, Michael A Cousin
May 24, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sung Hyun Kim, Timothy A Ryan
Feb 22, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Giselle Cheung, Michael A Cousin
Mar 26, 2014·The Journal of Cell Biology·Jaroslaw KasprowiczPatrik Verstreken
Apr 23, 2014·The Journal of Cell Biology·Katharina SchneiderBritta Qualmann
May 7, 2011·International Journal of Alzheimer's Disease·Karen Janet Smillie, Michael Alan Cousin
May 24, 2012·Journal of Biomedicine & Biotechnology·Monica VitaleNicola Zambrano
Jul 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Natasha K HussainRichard L Huganir
May 25, 2010·Cellular and Molecular Life Sciences : CMLS·Megan ChircopPhillip J Robinson
Nov 27, 2015·Neuron·Jessica C Nicholson-FishMichael A Cousin
Jun 23, 2007·The Journal of Physiology·Emma L ClaytonMichael A Cousin

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