Syndecan-4 proteoglycan cytoplasmic domain and phosphatidylinositol 4,5-bisphosphate coordinately regulate protein kinase C activity.

The Journal of Biological Chemistry
E S OhJ R Couchman

Abstract

Phosphatidylinositol 4,5-bisphosphate (PIP2) is involved in the organization of the actin cytoskeleton by regulating actin-associated proteins. The transmembrane heparan sulfate proteoglycan syndecan-4 also plays a critical role in protein kinase C (PKC) signaling in the formation of focal adhesions and actin stress fibers. The cytoplasmic domain of syndecan-4 core protein directly interacts with and potentiates PKCalpha activity, and it can directly interact with the phos- phoinositide PIP2. We, therefore, investigated whether the interaction of inositol phosphates and inositol phospholipids with syndecan-4 could regulate PKC activity. Data from in vitro kinase assays using purified PKCalpha beta gamma show that in the absence of phosphatidylserine and diolein, PIP2 increased the extent of autophosphorylation of PKCalpha beta gamma and partially activated it to phosphorylate both histone III-S and an epidermal growth factor receptor peptide. This activity was dose-dependent, and its calcium dependence varied with PKC isotype/source. Addition of the cytoplasmic syndecan-4 peptide, but not equivalent syndecan-1 or syndecan-2 peptides, potentiated the partial activation of PKCalpha beta gamma by PIP2, resulting in activity greate...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·P W Majerus
Jan 1, 1992·Annual Review of Cell Biology·M BernfieldE J Lose
Oct 1, 1992·Current Opinion in Cell Biology·M H GinsbergE F Plow
Jan 1, 1992·Advances in Experimental Medicine and Biology·A Woods, J R Couchman
Jun 1, 1991·Archives of Biochemistry and Biophysics·A ChauhanV P Chauhan
Aug 1, 1989·The Journal of Cell Biology·S JakenT Klauck
Jul 28, 1995·The Journal of Biological Chemistry·K F ToliasC L Carpenter
Sep 8, 1995·The Journal of Biological Chemistry·R H SohnP J Goldschmidt-Clermont
Jan 27, 1995·Cell·N Divecha, R F Irvine
Oct 1, 1995·Current Opinion in Cell Biology·K M Yamada, S Miyamoto
Nov 1, 1995·Molecular Biology of the Cell·P C Baciu, P F Goetinck
Jan 1, 1995·Annual Review of Cell and Developmental Biology·D A Schafer, J A Cooper
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Mar 28, 1997·The Journal of Biological Chemistry·E S OhJ R Couchman
Feb 1, 1995·Chemistry & Biology·P A Janmey
Nov 1, 1993·Trends in Cell Biology·L M Machesky, T D Poland
Oct 1, 1992·Trends in Cell Biology·M A Schwartz
Mar 1, 1996·Trends in Cell Biology·P R ShepherdH W Davidson

❮ Previous
Next ❯

Citations

Jul 27, 2000·Journal of Cellular Physiology·Y GaoM Simons
Apr 24, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Laura BriñasMarc Bitoun
Jul 15, 2009·Cell and Tissue Research·Xiaojie XianJohn R Couchman
May 17, 2003·Experimental Cell Research·DeannaLee M Beauvais, Alan C Rapraeger
May 9, 2001·Biochimica Et Biophysica Acta·S J SlaterC D Stubbs
Dec 1, 2001·Cellular Signalling·M Simons, A Horowitz
Jan 9, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·A WoodsJ R Couchman
Sep 7, 2001·Current Opinion in Cell Biology·A Woods, J R Couchman
Nov 18, 1998·Current Opinion in Cell Biology·A C Rapraeger, V L Ott
Jul 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bhavna ChananaGerd Vorbrüggen
Aug 30, 2008·The Journal of Biological Chemistry·James R WhitefordJohn R Couchman
Jun 3, 2011·Cardiovascular Research·Frank EchtermeyerGregor Theilmeier
Jan 26, 2011·Development, Growth & Differentiation·Yan SongSandra G Velleman
Oct 8, 1999·Alcoholism, Clinical and Experimental Research·C D Stubbs, S J Slater
Nov 19, 2013·Biochemistry. Biokhimii︠a︡·E I Leonova, O V Galzitskaya
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Nov 19, 2003·BMC Neuroscience·Laurence GoutebrozeJean-Antoine Girault
Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·DeannaLee M Beauvais, Alan C Rapraeger
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SaoncellaP F Goetinck
Jun 23, 2010·Annual Review of Cell and Developmental Biology·John R Couchman
Sep 11, 2001·Biochemical and Biophysical Research Communications·N C KaneiderC J Wiedermann
Jun 1, 2000·Leukemia & Lymphoma·L Kopper, A Sebestyén
Apr 16, 2003·The Journal of Cell Biology·Zohreh Mostafavi-PourMartin J Humphries
May 16, 2002·The Journal of Cell Biology·Arie HorowitzMichael Simons
Nov 1, 2015·Advanced Drug Delivery Reviews·Hinke A B MulthauptJohn R Couchman
Sep 4, 2012·The International Journal of Biochemistry & Cell Biology·E OkinaJ R Couchman
Nov 26, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yan SongSandra G Velleman
Jun 19, 2009·Advances in Enzyme Regulation·Danuta WawrzakPascale Zimmermann
Jul 16, 2008·European Journal of Cell Biology·Youngsil ChoiEok-Soo Oh
Jun 24, 2015·Biochemical and Biophysical Research Communications·Heeyoun KimWeontae Lee
Jun 23, 2009·Scandinavian Journal of Medicine & Science in Sports·E OkinaJ R Couchman
May 18, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C FeistritzerC J Wiedermann

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