Syndecan-4 regulates ATF-2 transcriptional activity in a Rac1-dependent manner.

The Journal of Biological Chemistry
S SaoncellaPaul F Goetinck

Abstract

Syndecan-4 is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. These cellular events are regulated by the small GTPase Rho, and in the absence of syndecan-4 ligation, cellular levels of GTP-bound Rho are decreased implicating syndecan-4 in the regulation of the small GTPases. In the present study we report that, compared with wild type cells, fibronectin-adherent syndecan-4-null fibroblasts showed enhanced lamellipodia and increased Rac1 activity that could be down-regulated by re-expression of syndecan-4 in the mutant cells. Consistent with the role for Rac1 in activating p38 and JNK signaling, syndecan-4-null cells display higher levels of active p38 MAPK and JNK that were abolished by the expression of a dominant-negative RacN17 mutant. Since p38 and JNK regulate gene expression by phosphorylating and activating transcription factors, we compared both the phosphorylation state and the transcriptional activity of the ATF-2 transcription factor, as a direct p38 and JNK target in syndecan-4-null and wild type cells. In the absence of syndecan...Continue Reading

References

May 1, 1997·Molecular and Cellular Biology·A J WhitmarshR J Davis
Feb 7, 1998·Science·A Hall
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Dec 18, 1998·The Journal of Biological Chemistry·V L Ott, A C Rapraeger
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SaoncellaP F Goetinck
May 18, 1999·The Journal of Biological Chemistry·Y ZhangM Simons
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Oct 13, 2000·Science·V S KraynovK M Hahn
Feb 13, 2001·The Journal of Clinical Investigation·F EchtermeyerP Goetinck
Apr 17, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·K IshiguroT Muramatsu
Oct 5, 2001·The Journal of Biological Chemistry·K IshiguroT Muramatsu
Jan 15, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Lei Li, Elliot L Chaikof
Feb 28, 2002·Nature Cell Biology·Miguel Angel Del PozoMartin Alexander Schwartz
Jun 28, 2002·The Journal of Biological Chemistry·Sarah A Wilcox-AdelmanPaul F Goetinck
Jul 9, 2002·Genes & Development·Anja Schmidt, Alan Hall
Sep 17, 2003·Glycoconjugate Journal·Sarah A Wilcox-AdelmanPaul F Goetinck
Feb 7, 2004·Science·Miguel A del PozoMartin A Schwartz

❮ Previous
Next ❯

Citations

Nov 1, 2007·Nature Reviews. Molecular Cell Biology·Mark R MorganMark D Bass
Jan 17, 2009·The Journal of Biological Chemistry·Claire M MahoneyMark D Bass
Jun 23, 2009·Scandinavian Journal of Medicine & Science in Sports·E OkinaJ R Couchman
May 11, 2012·International Journal of Cancer. Journal International Du Cancer·Sherif A IbrahimMartin Götte
Apr 5, 2016·Fertility and Sterility·Anca Chelariu-RaicuMartin Götte
Nov 28, 2013·The International Journal of Biochemistry & Cell Biology·Pablo AstudilloJuan Larraín
Sep 20, 2007·The Journal of Medical Investigation : JMI·Taro MutoTakafumi Noma
Mar 6, 2009·Cell Adhesion & Migration·Carlos Carmona-FontaineRoberto Mayor
May 6, 2006·Circulation Research·Eugene TkachenkoMichael Simons
Mar 19, 2005·Circulation Research·Eugene TkachenkoMichael Simons
Feb 5, 2005·The Journal of Biological Chemistry·Kristen A Johnson, Robert A Terkeltaub
Jul 27, 2007·American Journal of Physiology. Cell Physiology·Mario Barac-NietoAdrian Spitzer
Jul 23, 2005·The Journal of Biological Chemistry·Enzo CalauttiPaul F Goetinck
Feb 6, 2020·International Journal of Molecular Sciences·Daniel BecskyAniko Keller-Pinter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.