Syndrome Evaluation System for Simultaneous Detection Pathogens Causing Acute Encephalitic Syndrome in India, Part-1: Development and Standardization of the Assay

Frontiers in Medicine
Sunil GovekarRavikumar V Banda

Abstract

A large number of organisms are known to cause acute encephalitic syndrome (AES). A number of diagnostic tests have to be performed in order to arrive at a probable pathogen causing AES thus making it a very time consuming, laborious and expensive. The problem is further compounded by the lack of availability of sufficient volume of Cerebrospinal fluid (CSF). Thus, there is an urgent need of a diagnostic tool for the simultaneous detection of all probable pathogens responsible for causing AES. Here we report the development of a novel diagnostic method, Syndrome Evaluation System (SES) for the simultaneous detection of 22 pathogens including RNA and DNA Viruses, bacteria, fungi, and parasite all endemic to India and Southeast Asia in a single sample using a novel multiplexing strategy. Syndrome Evaluation System (SES) involves isolation of nucleic acid, multiplex amplification of the DNA, and cDNA followed by identification of the amplified product by sequence specific hybridization on SES platform with the final read out being a visually recordable colored signal. The total time required to carry out this diagnostic procedure is 7 h. The SES was standardized using the commercially available vaccines, panels and cell culture gr...Continue Reading

References

Oct 1, 1994·Journal of Clinical Microbiology·H A RotbartM Loeffelholz
Mar 1, 1997·Journal of Clinical Microbiology·S J ReadC R Bangham
Sep 2, 2000·Journal of Clinical Microbiology·G JohnsonR Tellier
Nov 29, 2001·Journal of Clinical Microbiology·P MarkoulatosJ Kourea-Kremastinou
Nov 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·David WangJoseph L DeRisi
Jan 9, 2003·Journal of Clinical Microbiology·Marie L LandryDavid Ferguson
Feb 24, 2004·Journal of Neurology, Neurosurgery, and Psychiatry·P G E Kennedy
Oct 19, 2004·Clinical Microbiology Reviews·Roberta L Debiasi, Kenneth L Tyler
Jun 30, 2005·Indian Journal of Pediatrics·Arun BansalS Ramesh
Feb 24, 2006·Emerging Infectious Diseases·Mandeep S ChadhaAkhilesh Mishra
May 12, 2006·Indian Journal of Medical Microbiology·V Ravi
Aug 30, 2006·Epidemiology and Infection·N KhetsurianiL J Anderson
Oct 6, 2006·Journal of Clinical Microbiology·Yvonne QvarnstromAlexandre J da Silva
Nov 7, 2006·Virology Journal·Ekta GuptaShobha Broor
Jan 29, 2008·Journal of the Neurological Sciences·Rashmi KumarV L Nag
Feb 6, 2009·Emerging Infectious Diseases·Gajanan N SapkalMilind M Gore
May 15, 2009·Neurology India·Nitin H ChandakHatim F Daginawala
Apr 7, 2011·Molecular and Cellular Probes·Nipaporn SankuntawViraphong Lulitanond

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Datasets Mentioned

BETA
M15240.1
AF212302.2
EF027139.1
GQ984141.1

Methods Mentioned

BETA
PCR
electrophoresis
nucleic acid extraction

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