Synergic Effects of CD40 and CD86 Silencing in Dendritic Cells on the Control of Allergic Diseases

International Archives of Allergy and Immunology
Motohiko SuzukiShinya Ozaki

Abstract

We previously reported that siRNA-induced CD40-silenced dendritic cells (DCs) inhibit allergic responses and symptoms. However, more potent therapies are needed. To our knowledge, synergic effects of gene silencing in DCs by ≥2 siRNAs have not been reported to control allergic diseases. Therefore, we investigated the synergistic effects of the silencing of CD40 and CD86 in DCs on allergic responses. Mice were treated with CD40/CD86-silenced DCs, which were transfected with CD40/CD86 siRNAs and pulsed with ovalbumin (OVA) antigen. The effects of these DCs on allergic symptoms and allergic responses were estimated. The administration of CD40/CD86-silenced OVA-pulsed DCs significantly inhibited the number of sneezes and nasal rubbing movements, the number of eosinophils in the nasal mucosa, and the level of OVA-specific IgE when compared with those for CD40- or CD86-silenced OVA-pulsed DCs alone (p < 0.01). These inhibitory effects were detected before sensitization as well as after the establishment of allergic rhinitis. CD40/CD86-silenced OVA-pulsed DCs did not inhibit KLH-induced allergies. Foxp3 gene expression was significantly upregulated in CD40-silenced DCs compared to in CD86-silenced DCs (p < 0.01). IL-4 production by T ...Continue Reading

Citations

May 9, 2019·American Journal of Rhinology & Allergy·Motohiko SuzukiShingo Murakami
Aug 28, 2021·International Journal of Molecular Sciences·Émile Bélanger, Catherine Laprise
Sep 24, 2021·Chinese Medical Journal·Chun-Hu WangYou-Bin Wang
Nov 7, 2019·International Archives of Allergy and Immunology·Daniëlle Verschoor, Stephan von Gunten

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