Synergie between molecular imprinted polymer based on solid-phase extraction and quartz crystal microbalance technique for 8-OHdG sensing

Biosensors & Bioelectronics
Arzu ErsözRidvan Say

Abstract

Recently, the 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been used as a marker to determine the oxidative stress. There is no any cheap and easy determination method based on chips and sensor systems for the determination of 8-OHdG. In this study, we have proposed imprinting methods for 8-OHdG recognition and determination using methacryloylamidohistidine-platinum(II) [MAH-Pt(II)] as a new metal-chelating monomer. The study includes the solid-phase extraction (SPE) of blood sample by a new 8-OHdG imprinted sorbent and the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor via ligand interaction. 8-OHdG imprinted sorbent has prepared by bulk polymerization of MAH-Pt(II) and N-N'-methylenebisacrylamide. 8-OHdG imprinted sensor has prepared on a QCM chip coating the thiol pretreated Au electrode. At the end of these steps, a thin molecular imprinted polymer (MIP) film for the detection of 8-OHdG has developed and analytical performance of QCM sensor which has prepared using MIP was investigated. The affinity constant (K(a)) for 8-OHdG using MAH-Pt-based thin film has determined by using the Scatchard method. The average percentage recovery of 8-OHdG from plasma samples was found as 80% by...Continue Reading

References

Mar 1, 1992·Enzyme and Microbial Technology·M PlomerB Hock
Jun 1, 1992·Molecular and Cellular Biology·K SatoH Endo
Jan 1, 1997·Biosensors & Bioelectronics·J L HarteveldE R Wils
Apr 11, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Akihiko KatoAkira Hishida
Jul 5, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Stacy D ArnettCraig E Lunte
Aug 16, 2005·Analytical Chemistry·Dar-Fu TaiLi-Kuang Chen
Oct 14, 2006·Analytical Chemistry·Ayça Atilir OzcanArzu Ersöz
Apr 26, 2007·Analytica Chimica Acta·Claudio BaggianiCristina Giovannoli
Aug 1, 2007·Free Radical Biology & Medicine·Roberto CangemiFrancesco Violi

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Citations

Dec 11, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shian ZhongYan Wang
Oct 22, 2011·International Journal of Molecular Sciences·Giuseppe VasapolloGiuseppe Mele
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Mar 30, 2017·Sensors·Giulia Selvolini, Giovanna Marrazza
Sep 28, 2010·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Stéphanie LagaduVirginie Prevost
Sep 14, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chien-Hui SuGou-Jen Wang
Mar 24, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ahmad MobedGhazal Koohkan

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