Synergism between cryoablation and GM-CSF: enhanced immune function of splenic dendritic cells in mice with glioma

Neuroreport
Hongchao XuShizhong Zhang

Abstract

Glioma is the most common malignant primary brain tumor, and it has a poor prognosis. Studies have shown that cryoablation can activate antitumor immunoeffects by promoting the augmentation of dendritic cells (DCs). Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to be useful for immunotherapy against glioma because it can stimulate DCs to present tumor antigen. Previous studies have shown that cryoablation and GM-CSF can exert antitumor effects. To test the hypothesis that combined therapy with cryoablation and GM-CSF for glioma could synergistically improve specific antiglioma immunity in mice, we tested the validity of this assumption in a murine subcutaneous GL261 glioma model. C57BL/6 mice with subcutaneous GL261 glioma were created and divided into four groups: no treatment, GM-CSF injection, cryoablation treatment, and GM-CSF and cryoablation combined treatment (n=20 in each group). Serial immune indicators were detected at sequential time points during treatment. Compared with the other groups, in the combined treatment group, DCs were more activated and their numbers were markedly upregulated, the secretion of interferon-γ from Th1 cells of mice spleen was increased, and the cytolytic activity ...Continue Reading

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Citations

Jun 28, 2017·World Journal of Gastroenterology : WJG·Qiao-Huan YanYing-Gang Lv
Aug 9, 2020·Nature Reviews. Drug Discovery·Alexandre HarariLana E Kandalaft
Feb 26, 2020·Cryobiology·Jibing ChenKecheng Xu

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
flow cytometry
ELISA
magnetic
enzyme-linked immunosorbent assay

Software Mentioned

SPSS

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