Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells

Journal of Biomechanics
Chia-Ching WuB Linju Yen

Abstract

There have been intensive studies on the differentiation of endothelial progenitor cells (EPCs) into endothelial cells. We investigated the endothelial differentiation of placenta-derived multipotent cells (PDMCs), a population of CD34(-)/CD133(-)/Flk-1(-) cells. PDMCs were cultured in basal media or media containing endothelial growth factors (EGM), including vascular endothelial growth factor (VEGF), for 3 days and then subjected to shear stress of 6 or 12dyn/cm(2) for 24h. Culture of PDMCs in EGM under static conditions resulted in significant increases in VEGF receptor-1 (Flt-1) and receptor-2 (Flk-1) expression. Application of shear stress at 12dyn/cm(2) to these cells led to significant increases in their expression of von Willebrand Factor and platelet-endothelial cell adhesion molecule-1 at both the gene and protein levels. Shear stress at 6dyn/cm(2) had lesser effects. Uptakes of acetylated low-density lipoproteins as well as formation of tube-like structures on Matrigel were significantly increased after subjecting to shear stress of 12dyn/cm(2) for 24h. Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs.

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Citations

Apr 17, 2009·Biomedical Microdevices·Huei-Wen WuGwo-Bin Lee
May 7, 2009·Acta Pharmacologica Sinica·Jian-de DongJian Zhang
Feb 8, 2011·Antioxidants & Redox Signaling·Luigi Adamo, Guillermo García-Cardeña
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Apr 10, 2010·Journal of Biomedicine & Biotechnology·Igor TitushkinMichael Cho
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Jun 29, 2012·Journal of Cellular Biochemistry·Bin-Bin ChengZong-Lai Jiang
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