Synergism of interleukin 12, interleukin 3 and serum factor on primitive human hematopoietic progenitor cells

Stem Cells
A HiraoA Makimoto

Abstract

Interleukin 12 (IL-12), or natural killer cell stimulatory factor (NKSF), has multiple biologic effects on T lymphocytes and natural killer cells. In this study, we evaluated the effect of IL-12 on human hematopoiesis by analyzing the growth of CD34+33- cells in methylcellulose culture with or without serum. Blood cells were collected by apheresis from an expanded stem cell pool during the rebound phase of hematopoiesis after chemotherapy and purified by combined purification procedures to yield target CD34+33- cells. IL-12, by itself or in combination with IL-3 and erythropoietin (Epo), had no effect on the formation of colonies from the unfractionated blood mononuclear cells. In the presence of fetal bovine serum (FBS), IL-12 increased the number of colony forming units for granulocyte-macrophage (CFU-GM) from CD34+33- cells which responded to IL-3, but not the number of CFU-GM from those which responded to stem cell factor (SCF) or granulocyte colony stimulating factor (G-CSF). This effect was not abolished by the addition of monoclonal antibodies against tumor necrosis factor alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-1 alpha, IL-6, G-CSF, or granulocyte/macrophage CSF (GM-CSF). However, the stimulatory effect of I...Continue Reading

References

Feb 1, 1992·Immunology Today·D M MerbergS C Clark
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R BonoM Fellous
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K IkebuchiM Ogawa

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Citations

Sep 1, 1995·Research in Immunology·S E Jacobsen
Jan 27, 1998·Hematology/oncology Clinics of North America·O P VeibyH R Snodgrass
Oct 31, 1996·Annals of the New York Academy of Sciences·M T Lotze

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