Synergistic actions of glucagon and miniglucagon on Ca2+ mobilization in cardiac cells

Circulation Research
A SauvadetC Pavoine

Abstract

It has been recently shown that the physiological processing of glucagon into its C-terminal (19-29) fragment, miniglucagon, by cardiac cells was essential for the contractile positive inotropic effect of the hormone. However, the mechanisms underlying the effects of miniglucagon remained undetermined. In the present study, we assessed the effects of miniglucagon on Ca2+ homeostasis in embryonic chick ventricular myocytes. In quiescent cells, short-term applications of 0.1 nmol/L miniglucagon markedly increased the accumulation of 45Ca into intracellular compartments resistant to digitonin lysis and sensitive to caffeine. Ca2+ accumulation into the sarcoplasmic reticular (SR) store was further attested by fura 2 imaging studies on quiescent or prestimulated cells: miniglucagon potentiated Ca2+ release from the SR compartment triggered by caffeine and evoked a rise in cytosolic Ca2+ when applied on cells pretreated with 1 mumol/L thapsigargin, a specific inhibitor of the SR Ca2+ pump. Glucagon alone produced a small cytosolic Ca2+ signal that was considerably amplified by miniglucagon. The action of glucagon was mimicked by 8-bromo-cAMP and was blocked by isradipine, suggesting that it relied on the activation of L-type Ca2+ cha...Continue Reading

References

May 1, 1991·The American Journal of Physiology·C PavoineF Pecker
Nov 1, 1989·Journal of Biochemistry·T TakasagoM Shigekawa
Jun 12, 1987·Biochimica Et Biophysica Acta·T H KuoJ Wiener
Mar 1, 1988·Critical Care Medicine·A L ZaritskyB Chernow
Sep 30, 1971·The New England Journal of Medicine·W W Parmley
May 1, 1994·Cardiovascular Drugs and Therapy·T Godfraind

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Citations

Aug 14, 1999·Progress in Biophysics and Molecular Biology·Y Shimoni
Jun 18, 2004·Heart·K Chang-ChretienD P Judge
Sep 30, 2003·Journal of Toxicology. Clinical Toxicology·Benoit Bailey
Dec 19, 2014·Endocrinology, Diabetes & Metabolism Case Reports·Kun ZhangTorsten K Roepke
Mar 10, 2001·The Journal of Thoracic and Cardiovascular Surgery·T A FolliguetS N Hatem
Aug 27, 2005·Human & Experimental Toxicology·D M WoodP I Dargan
Mar 17, 2018·The Journal of Clinical Endocrinology and Metabolism·Kasper Meidahl PetersenMikkel Bring Christensen
Jan 22, 2003·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Steven D Salhanick, Michael W Shannon
May 15, 2003·Endocrinology·Robert GrosMansoor Husain
May 9, 1997·The Journal of Biological Chemistry·A SauvadetC Pavoine
May 9, 2002·American Journal of Physiology. Cell Physiology·Aïssata AmadouFrançoise Pecker
Feb 25, 2005·The Journal of Biological Chemistry·Bouziane Ait-MamarCatherine Pavoine
Oct 21, 2004·Cellular Signalling·Catherine Pavoine, Nicole Defer

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