PMID: 2512012Dec 1, 1989

Synergistic activation by the glutamine-rich domains of human transcription factor Sp1

Cell
A J CoureyR Tjian

Abstract

We have examined the role of protein-protein interactions in modulating the activity of Sp1, a human transcription factor that utilizes glutamine-rich activation domains. These domains may represent a commonly used structural motif, since a nonhomologous glutamine-rich segment from the Drosophila Antennapedia protein is also a potent activator when fused to the Sp1 DNA binding domain. Sp1 is generally considered a proximal promoter factor that can only stimulate transcription when bound close to the initiation site. However, here we present evidence that distally and proximally bound Sp1 can stimulate transcription synergistically. In addition, a DNA binding-deficient mutant of Sp1 that retains glutamine-rich domains can interact with proximally bound Sp1 to superactivate transcription. Glutaraldehyde cross-linking provides direct evidence for an interaction between Sp1 monomers. Thus, Sp1-Sp1 interactions may play an important role in modulating promoter activity.

References

Mar 1, 1996·Journal of Molecular Recognition : JMR·D Henderson, L H Hurley
Sep 5, 2002·Journal of Cellular Biochemistry·Donald C WilkersonSidney R Grimes
Oct 1, 1994·Protein Science : a Publication of the Protein Society·M F Perutz
Jul 1, 1990·Somatic Cell and Molecular Genetics·H P MüllerW Schaffner
Aug 31, 2000·Molecular Neurobiology·X J Li
Jan 1, 1990·Molecular Neurobiology·K M Walton, R P Rehfuss
Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Mar 1, 1995·Pharmaceutica acta Helvetiae·M F Perutz
Oct 30, 1996·Molecular and Biochemical Parasitology·B ConnollyD F Smith
Nov 1, 1991·Mechanisms of Development·T el-Baradi, T Pieler
Jun 1, 1990·Current Opinion in Cell Biology·J T Kadonaga
Mar 22, 2005·Gene·Shijian Chu, Thomas J Ferro
Oct 3, 2002·Biochemical and Biophysical Research Communications·Jürgen NeesenIrene Mendoza-Lujambio
Mar 21, 1992·Journal of Theoretical Biology·R Nussinov
Sep 6, 2002·Molecular Immunology·So-Youn ParkTae-Hee Han
Oct 2, 2002·Molecular and Cellular Endocrinology·Peter Bouwman, Sjaak Philipsen
Nov 24, 2001·Brain Research Bulletin·B L KaganR Azimova
Nov 26, 1999·Gene·G Suske

Citations

Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S P Jackson, R Tjian
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
Jul 1, 1988·Cell·W Driever, C Nüsslein-Volhard
Aug 25, 1988·Nature·G Gill, M Ptashne

Related Concepts

Transfection
Antp
Transcription, Genetic
Transcription Initiation
Promoter
Drosophila
Antennapedia Homeodomain Protein
Protein Domain
DNA Binding Domain
Mutant

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