Synergistic activation of adenylyl cyclase is dependent upon phospholipase C-mediated processes in human neuroblastoma SK-N-BE(2)C cells

European Journal of Pharmacology
B C SuhK T Kim

Abstract

1-[6-[17 beta-3-Methoxyestra-1,3.5(10)-trien-17-yl]amino]hexyl]-1 H-pyrrole-2,5-dione (U-73122), an inhibitor of processes involved in the activation of phospholipase C, was used to assess the role of phospholipase C activation in the synergistic elevation of cAMP induced by carbachol and prostaglandin E2 in human neuroblastoma (SK-N-BE(2)C cells. Pre-treatment of the cells with U-73122 resulted in inhibition of carbachol-induced intracellular Ca2+ ([Ca2+]i) rise and inositol 1,4,5-trisphosphate (InsP3) generation, with maximal and half maximal inhibition (IC50) occurring at approximately 15 microM and 3.2 microM, respectively. U-731222 also inhibited the synergistic enhancement of cAMP accumulation induced by carbachol and prostaglandin E2 in a concentration-dependent manner with maximum and IC50 at 12 +/- 4 microM and 3.4 +/- 0.3 microM, respectively. However, U-73122 did significantly inhibit prostaglandin E2-induced production. While 1,2-bis(o-aminophenoxy)ethane-N,N,N,'N'-tetraacetic acid (BAPTA/AM) treatment decreased the synergistic cAMP accumulation by 28%m addition U-73122 further decreased it down to complete inhibition. Furthermore, GTP gamma S- and A1F4(-)-induced InsP3 generation in digitonin-mediated permeabilized...Continue Reading

References

Sep 1, 1992·Trends in Pharmacological Sciences·J Baumgold
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Yoshimura, D M Cooper
Sep 18, 1992·Cell·W J Tang, A G Gilman
Jul 15, 1991·Biochemical and Biophysical Research Communications·Y Nakagawa-YagiM Takayama
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P G FeinsteinR R Reed
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B N Gao, A G Gilman
Aug 15, 1988·Biochemical and Biophysical Research Communications·J Baumgold, P H Fishman
Sep 15, 1988·The Biochemical Journal·D M Cooper, K K Caldwell
Jun 1, 1993·Pharmacology & Therapeutics·M P Caulfield
May 12, 1995·The Journal of Biological Chemistry·K HellevuoB Tabakoff
Apr 1, 1995·Trends in Biochemical Sciences·J IngleseR J Lefkowitz
Aug 29, 1994·FEBS Letters·N DeferJ Hanoune
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Iyengar
Jan 1, 1993·Journal of Neurochemistry·Z XiaD R Storm

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Citations

Dec 18, 2001·Cell Motility and the Cytoskeleton·B Safiejko-Mroczka, P B Bell
Dec 11, 2002·Biochemical Pharmacology·Tae-Ju ParkKyong-Tai Kim
Mar 8, 2002·Cellular Signalling·Eun Mi Hur, Kyong Tai Kim
Mar 29, 2001·Biochemical Pharmacology·T J ParkK T Kim
Jun 14, 2002·Archives of Biochemistry and Biophysics·Li-jun ShiChun-an Wang
Mar 18, 2003·Cellular Signalling·Tae-Ju Park, Kyong-Tai Kim

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