Abstract
1-[6-[17 beta-3-Methoxyestra-1,3.5(10)-trien-17-yl]amino]hexyl]-1 H-pyrrole-2,5-dione (U-73122), an inhibitor of processes involved in the activation of phospholipase C, was used to assess the role of phospholipase C activation in the synergistic elevation of cAMP induced by carbachol and prostaglandin E2 in human neuroblastoma (SK-N-BE(2)C cells. Pre-treatment of the cells with U-73122 resulted in inhibition of carbachol-induced intracellular Ca2+ ([Ca2+]i) rise and inositol 1,4,5-trisphosphate (InsP3) generation, with maximal and half maximal inhibition (IC50) occurring at approximately 15 microM and 3.2 microM, respectively. U-731222 also inhibited the synergistic enhancement of cAMP accumulation induced by carbachol and prostaglandin E2 in a concentration-dependent manner with maximum and IC50 at 12 +/- 4 microM and 3.4 +/- 0.3 microM, respectively. However, U-73122 did significantly inhibit prostaglandin E2-induced production. While 1,2-bis(o-aminophenoxy)ethane-N,N,N,'N'-tetraacetic acid (BAPTA/AM) treatment decreased the synergistic cAMP accumulation by 28%m addition U-73122 further decreased it down to complete inhibition. Furthermore, GTP gamma S- and A1F4(-)-induced InsP3 generation in digitonin-mediated permeabilized...Continue Reading
References
May 1, 1992·Journal of Neurochemistry·J BaumgoldG Fiskum
Sep 1, 1992·Trends in Pharmacological Sciences·J Baumgold
Aug 1, 1992·Neuron·B Hille
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Yoshimura, D M Cooper
Sep 18, 1992·Cell·W J Tang, A G Gilman
Jul 21, 1992·Biochemistry·E J ChoiD R Storm
Nov 12, 1991·Biochimica Et Biophysica Acta·C C JanssonK E Akerman
Apr 11, 1991·Nature·S J TaylorJ H Exton
Jul 15, 1991·Biochemical and Biophysical Research Communications·Y Nakagawa-YagiM Takayama
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P G FeinsteinR R Reed
Feb 15, 1991·Science·A V SmrckaP C Sternweis
Jan 1, 1991·Methods in Enzymology·Y Salomon
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B N Gao, A G Gilman
Dec 6, 1991·Science·W J Tang, A G Gilman
Jan 15, 1990·The Biochemical Journal·R A ChallissS R Nahorski
Sep 18, 1990·European Journal of Pharmacology·A L WillcocksS R Nahorski
Sep 1, 1986·The Biochemical Journal·C P DownesR F Irvine
Aug 15, 1988·Biochemical and Biophysical Research Communications·J Baumgold, P H Fishman
May 7, 1987·Nature·T YoshimasaM G Caron
Sep 15, 1988·The Biochemical Journal·D M Cooper, K K Caldwell
Dec 15, 1988·The Biochemical Journal·F Di VirgilioT H Steinberg
Jun 1, 1993·Pharmacology & Therapeutics·M P Caulfield
Mar 2, 1993·Biochemistry·E J ChoiD R Storm
May 12, 1995·The Journal of Biological Chemistry·K HellevuoB Tabakoff
Jun 1, 1995·Journal of Neurochemistry·B C Suh, K T Kim
Apr 1, 1995·Trends in Biochemical Sciences·J IngleseR J Lefkowitz
Aug 29, 1994·FEBS Letters·N DeferJ Hanoune
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Iyengar
Jan 1, 1993·Journal of Neurochemistry·Z XiaD R Storm
Citations
Dec 18, 2001·Cell Motility and the Cytoskeleton·B Safiejko-Mroczka, P B Bell
Dec 11, 2002·Biochemical Pharmacology·Tae-Ju ParkKyong-Tai Kim
Aug 28, 1998·European Journal of Pharmacology·S Byung-ChangK Kyong-Tai
Jan 22, 2003·Journal of Immunological Methods·Waldemar GonsiorekR William Hipkin
Mar 8, 2002·Cellular Signalling·Eun Mi Hur, Kyong Tai Kim
Apr 20, 2013·Science·Nozomu KonoHiroyuki Arai
Mar 29, 2001·Biochemical Pharmacology·T J ParkK T Kim
Oct 26, 2001·Journal of Neurochemistry·S Y ChoiK T Kim
Jun 14, 2002·Archives of Biochemistry and Biophysics·Li-jun ShiChun-an Wang
Mar 18, 2003·Cellular Signalling·Tae-Ju Park, Kyong-Tai Kim
Jun 23, 2009·Vascular Pharmacology·Tomohiro MiikeKazuyoshi Kurahashi