Abstract
Several DNA binding NF-kappaB subunits are substrates for cGMP-dependent kinase (PKG) and their transactivation from cognate sites is induced by phosphorylation. This includes p50, which does not have a transcriptional activation domain and therefore needs to bind to other proteins to mediate gene expression. Here, we describe the synergistic transactivation by p50 and PKG from the CMV promoter. This is caused not only by phosphorylation of p50, leading to increased DNA binding, but also by PKG-dependent activation of CRE sites in the promoter. One of the CRE sites is located directly adjacent to a NF-kappaB site and is essential for p50-mediated induction of transcription. According to the binding of CREB to p50 in pull-down assays and according to the inhibition of p50-dependent transactivation by dominant-negative CREB, this reflects the formation of a transcription factor complex containing CREB and p50. The nuclear translocation of NF-kappaB is insufficient to distinguish among the multitude of promoters that harbor cognate recognition sites. The phosphorylation of multiple transcription factors by an upstream kinase, such as PKG, can lead to the formation of transcription factor complexes and differential transactivation ...Continue Reading
References
Mar 25, 1991·Nucleic Acids Research·O Bakker, M G Parker
Dec 1, 1990·Molecular and Cellular Biology·E A ParkR W Hanson
Jan 1, 1990·The Journal of General Virology·T StammingerB Fleckenstein
Apr 28, 1989·Science·S L CrossW J Leonard
Apr 1, 1995·Immunity·G F WeberH Cantor
Mar 12, 1993·Cell·V BoursU Siebenlist
Mar 1, 1996·The Journal of Biological Chemistry·T GudiR B Pilz
Aug 1, 1996·Trends in Genetics : TIG·L A Stargell, K Struhl
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A MontanoG P Nolan
Jul 14, 1998·Molecular Cell·H ZhongS Ghosh
Feb 20, 1999·The Journal of Biological Chemistry·K A DooleyT F Osborne
Jan 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·Y S ChangJ M Tarbell
Jun 30, 2000·Virology·S PröschW D Döcke
Aug 28, 2001·The Journal of Biological Chemistry·S PröschD H Krüger
Jan 10, 2002·Nature·B LemonR Tjian
Apr 5, 2002·Molecular Cell·Haihong ZhongSankar Ghosh
May 30, 2002·Virology·Martha J LewisWilliam W Hall
Jun 26, 2002·The Journal of Biological Chemistry·Darren E CasteelRenate B Pilz
Jul 18, 2002·The Journal of Biological Chemistry·Tanima GudiRenate B Pilz
Dec 18, 2003·The Journal of Biological Chemistry·Shunhui ZhuangRenate B Pilz
Citations
Jul 17, 2008·Nucleic Acids Research·Christina Saeten FjeldboTorunn Bruland
Dec 27, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·James P MaloneyJames E Loyd
Sep 9, 2006·BMC Developmental Biology·Michael MelnickTina Jaskoll
Dec 15, 2007·Retrovirology·Jia Hai LeeKuan-Teh Jeang
May 5, 2012·PloS One·Julie AmyotVincent Poitout
Jan 23, 2015·Biotechnology Journal·Adam J BrownDavid C James
Feb 2, 2006·Virus Research·Jasper M BoomkerMartin C Harmsen
Jun 5, 2008·Cancer Letters·Georg F Weber
Feb 25, 2014·FEBS Letters·Khalid KhanMinoru Yoshida
Dec 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura MeloniRudi Beyaert
Sep 24, 2009·Journal of Receptor and Signal Transduction Research·Dong-Qing WenJin-Bo Xu
Jan 21, 2021·Plasmid·Kyle SpivackJacob Elmer
Jul 22, 2021·Chemical Communications : Chem Comm·Zhenqiang FanKai Zhang
Jan 19, 2008·Archives of Oral Biology·Tina JaskollMichael Melnick