Synergistic anticancer activity of dietary tea polyphenols and bleomycin hydrochloride in human cervical cancer cell: Caspase-dependent and independent apoptotic pathways

Chemico-biological Interactions
Ali A AlshatwiRamesh Elango

Abstract

Bleomycin is a chemotherapeutic agent that is frequently used in the treatment of various cancers. Bleomycin causes serious adverse effects via antioxidant defense abnormalities against reactive oxygen species (ROS). However, the current cervical cancer monodrug therapy strategy has failed to produce the expected outcomes; hence, combinational therapies are gaining great interest. Tea polyphenols are also effective antioxidative and chemo-preventive agents. However, the combined effect of tea polyphenol (TPP) and bleomycin (BLM) against cervical cancer remains unknown. In this study, we focused on the potential of TPP on BLM anticancer activity against cervical cancer cells. Cervical cancer cells (SiHa) were treated with various concentrations of TPP, BLM and TPP combined with BLM (TPP-BLM), and their effects on cell growth, intracellular reactive oxygen species, poly-caspase activity, early apoptosis and the expression of caspase-3, caspase-8 and caspase-9, Bcl-2 and p53 were assessed. The MTT assay revealed that the SiHa cells were less sensitive to growth inhibition by TPP treatment compared with both BLM and the combination therapy. Nuclear staining indicated that exposure to TPP-BLM increased the percentage of apoptotic nu...Continue Reading

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Citations

Mar 8, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Jiaqi WangYingbin Liu
Apr 7, 2018·Critical Reviews in Food Science and Nutrition·Nevin SanlierIlker Atik
Sep 27, 2018·Journal of Food Science·Liyue LiuWei Liu
Sep 13, 2019·Critical Reviews in Food Science and Nutrition·Haroon KhanHui Cao
Feb 21, 2019·Journal of Cellular Physiology·Yang LinXianjun Meng
Dec 11, 2019·International Journal of Molecular Sciences·Guo-Yi TangHua-Bin Li
Feb 12, 2017·BMC Complementary and Alternative Medicine·S KhazaeiP Ismail
Sep 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying-Qi WangQing-Sheng Li
Oct 28, 2019·International Journal of Molecular Sciences·Xiangbing MaoJun He
Aug 31, 2019·Oxidative Medicine and Cellular Longevity·Serena GalatiAnnamaria Buschini
Jan 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Emanuelly Bernardes-OliveiraJanaina Cristiana de Oliveira Crispim
Nov 21, 2020·Journal of the American College of Nutrition·Saptadip Samanta
Apr 30, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nouf Abdulkareem Omer AlkharashiAli A Alshatwi

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