Synergistic Anticancer Effects of Formononetin and Temozolomide on Glioma C6 Cells

Biological & Pharmaceutical Bulletin
Xiong ZhangYingbin Li

Abstract

Temozolomide (TMZ) is currently the first-line drug used for clinical postoperative or non-surgical chemotherapy for glioma, but acquired and intrinsic resistance to TMZ limits its application. The anti-proliferative effect of formononetin on human glioma cells had been confirmed. To improve therapeutic effects of TMZ, we studied the effect of formononetin in combination with TMZ on C6 glioma cells. The anti-proliferative effect of C6 cells was tested by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. The synergy was evaluated by Chou-Talalay method. Morphological changes were observed by hematoxylin-eosin (HE) staining. The effect of formononetin in combination with TMZ on apoptosis of C6 cells was investigated by flow cytometry. The effect of formononetin in combination with TMZ on migration of cells was investigated by wound healing assay and transwell assay. The expression of proteins related to apoptosis and migration were detected by Western blot. These results showed that formononetin or TMZ alone could inhibit the growth of C6 cells in dose-dependent manner and formononetin in combination with TMZ had synergy effect on C6 cells. Further changes in cell morphology could be observed in drug combina...Continue Reading

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Citations

May 6, 2019·Cancers·Samantha Kah Ling OngAnupam Bishayee
Apr 1, 2019·Investigational New Drugs·Jian-Ning YaoLian-Feng Zhang
Aug 14, 2019·Frontiers in Pharmacology·Kai-Ching TayBey-Hing Goh
Nov 13, 2018·Cell Death Discovery·Adel Rezaei MoghadamJoseph W Gordon
Mar 23, 2021·Journal of Ethnopharmacology·Jia-Hui LiYing-Bin Li
Jul 24, 2021·Nutrition and Cancer·Afshin KaramiAmir Kiani

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