Synergistic antiproliferative and apoptotic effects induced by mixed epidermal growth factor receptor inhibitor ZD1839 and nitric oxide donor in human prostatic cancer cell lines

The Prostate
Murielle MimeaultJean-Pierre Hénichart

Abstract

The specific inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase, ZD1839 induces potent antitumoral effects on several advanced cancer types. The present study was undertaken to determine whether the combination of ZD1839 with an agent donating nitric oxide (NO(*)), sodium nitroprusside (SNP) results in a synergy of anticarcinogenic responses on metastatic prostate cancer (PC) cells. The antiproliferative and apoptotic/necrotic effects of ZD1839 and SNP alone or in combination were estimated on EGF- and serum-stimulated LNCaP, DU145, and PC3 cells by MTT growth tests, trypan blue dye exclusion method, and flow cytometric analyses. Moreover, the cellular ceramide levels were evaluated by the diacylglycerol kinase enzymatic method and the amounts of cytosolic cytochrome c by ELISA assays. ZD1839 and SNP alone or in combination at lower concentrations induced an inhibition of EGF- and serum-stimulated growth of LNCaP, DU145, and PC3 concomitant with an arrest in the G1 phase of cellular cycle. Interestingly, the mixed ZD1839 and SNP also caused a more substantial apoptotic/necrotic death of these PC cells as compared to drugs alone. Moreover, we have observed that an inhibition of acidic sphingomyelinase, hydrogen...Continue Reading

References

Aug 22, 1997·The Journal of Biological Chemistry·A Quillet-MaryG Laurent
Mar 6, 1999·The Journal of Biological Chemistry·A HuwilerH van den Bosch
Mar 26, 1999·Methods and Findings in Experimental and Clinical Pharmacology·M RubensteinP Guinan
May 1, 1999·Biochemical and Biophysical Research Communications·A HuwilerJ Pfeilschifter
Aug 28, 1999·Bioorganic & Medicinal Chemistry Letters·Y HouP G Wang
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·J IgarashiT Michel
Dec 22, 1999·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·X F LuY C Wong
Feb 19, 2000·Biochemical and Biophysical Research Communications·D E ModrakR Blumenthal
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·A GewiesM B Cohen
Nov 30, 2000·Biochemical and Biophysical Research Communications·Y S ChoY S Cho-Chung
Feb 13, 2001·Trends in Pharmacological Sciences·M GuzmánC Sánchez
Mar 20, 2001·Critical Reviews in Oncology/hematology·X D YangC G Davis
Jul 10, 2001·Archives of Andrology·N TanjiM Yokoyama
Aug 15, 2001·Urology·A KirschenbaumA C Levine
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangT Okamoto
Oct 24, 2001·Current Opinion in Oncology·E S KimR S Herbst
Mar 20, 2002·Nature Reviews. Cancer·B J Feldman, D Feldman
Mar 21, 2002·Expert Opinion on Therapeutic Targets·Jeremy R Graff
May 2, 2002·Bioorganic & Medicinal Chemistry·Xuejun WuPeng George Wang
May 16, 2002·The Journal of Biological Chemistry·Gerrit van Meer, Quirine Lisman
May 29, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·B Ogretmen, Y A Hannun
May 31, 2002·Expert Opinion on Investigational Drugs·Roy S Herbst
Jun 8, 2002·Biochemical and Biophysical Research Communications·Rebeca López-MarureLuis Felipe Montaño
Jun 20, 2002·Journal of Immunological Methods·Maria CastedoGuido Kroemer
Aug 31, 2002·The Oncologist·Malcolm Ranson

❮ Previous
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Citations

Dec 7, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G Aparicio GallegoL M Antón Aparicio
Mar 9, 2010·Molecular Pharmaceutics·Ellen V StevensMark H Schoenfisch
May 4, 2007·The Cancer Journal·Susan E Bates, Richard L Piekarz
Sep 25, 2015·Journal of Inorganic Biochemistry·Imalka MunaweeraKenneth J Balkus
Oct 3, 2006·International Journal of Cancer. Journal International Du Cancer·Murielle MimeaultSurinder K Batra
Jun 19, 2009·Medicinal Research Reviews·Yong LiPamela J Russell
Feb 21, 2006·Molecular Genetics and Metabolism·Ilaria BellezzaAlba Minelli
Dec 14, 2018·Journal of Biomaterials Science. Polymer Edition·Jilan WangChengkun Qin
Mar 23, 2017·Antioxidants & Redox Signaling·Amy J BurkeSharon A Glynn
Mar 16, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M Mimeault, S K Batra
Sep 17, 2009·Journal of Natural Products·Rong-Jyh LinChung-Yi Chen
Aug 28, 2021·European Journal of Medicinal Chemistry·Osama M SoltanMohamed Abdel-Aziz
Mar 30, 2006·Journal of Agricultural and Food Chemistry·Luciane A SaviClaudia M O Simões

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