Synergistic antitumoral effect of vinblastine and HSV-Tk/GCV gene therapy mediated by albumin-associated cationic liposomes

Journal of Controlled Release : Official Journal of the Controlled Release Society
H FanecaM C Pedroso de Lima

Abstract

Despite recent advances in conventional therapeutic approaches for cancer, the frequently observed acquired drug resistance and toxic side effects have limited their clinical application. The main goal of this work was to investigate the combined antitumoral effect of vinblastine with HSV-Tk/GCV "suicide" gene therapy mediated by human serum albumin (HSA)-associated lipoplexes, in mammary adenocarcinoma cells (TSA cells). Our results show that, among the different lipoplex formulations tested, HSA-associated complexes prepared from EPOPC:Chol liposomes, at the (4/1) (+/-) charge ratio, was the most efficient to mediate gene delivery, even in the presence of serum. The simultaneous addition of vinblastine and HSA-EPOPC:Chol/DNA (+/-) (4/1) lipoplexes to TSA cells improved transgene expression more than 10 times. When combined with the HSV-Tk/GCV "suicide" gene therapy mediated by HSA-EPOPC:Chol/DNA (+/-) (4/1) lipoplexes, vinblastine induced a great enhancement in the antitumoral activity in TSA cells. Most importantly, this combined strategy resulted in a significant synergistic effect, thus allowing the use of a much lower dose of the drug to achieve the same therapeutic effect. Overall, our results indicate that this approach...Continue Reading

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Citations

Oct 1, 2013·Journal of Biomaterials Science. Polymer Edition·Yuta NakamuraYoshiki Katayama
Jul 26, 2014·International Journal of Nanomedicine·Marta PassadouroHenrique Faneca
May 29, 2012·Cancer Letters·Sónia DuarteValérie Pierrefite-Carle
Jul 23, 2014·International Journal of Pharmaceutics·Dina FarinhaHenrique Faneca
May 26, 2015·Advanced Drug Delivery Reviews·Zahra KarjooArash Hatefi
Aug 24, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tetsuro TomiyamaYoshiki Katayama
Dec 17, 2009·Biochimica Et Biophysica Acta·Adriana O SantosJoão N Moreira
Jul 22, 2011·Biomacromolecules·M José MouraM Margarida Figueiredo
Apr 4, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohamed GaberAhmed Elzoghby
Mar 30, 2019·Nanomedicine·Masoud Mohseni-DargahBabak Kaboudin
Sep 6, 2014·Biofabrication·Filipa A M M GonçalvesJorge F J Coelho
Feb 22, 2021·Drug Discovery Today·Sarwar BegHani Choudhry
Jul 20, 2021·Chemical Reviews·Yuebao ZhangYizhou Dong
Mar 9, 2015·ACS Biomaterials Science & Engineering·Rumiana KoynovaRobert C MacDonald
May 31, 2011·Biochimie·Xiao-Xiang ZhangMark W Grinstaff

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