Synergistic chondroprotective effect of alpha-tocopherol, ascorbic acid, and selenium as well as glucosamine and chondroitin on oxidant induced cell death and inhibition of matrix metalloproteinase-3--studies in cultured chondrocytes.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Anne-Christi GraeserGerald Rimbach

Abstract

Overproduction of reactive oxygen species and impaired antioxidant defence accompanied by chronic inflammatory processes may impair joint health. Pro-inflammatory cytokines such as interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) stimulate the expression of metalloproteinases which degrade the extracellular matrix. Little is known regarding the potential synergistic effects of natural compounds such as alpha-tocopherol (alpha-toc), ascorbic acid (AA) and selenium (Se) on oxidant induced cell death. Furthermore studies regarding the metalloproteinase-3 inhibitory activity of glucosamine sulfate (GS) and chondroitin sulfate (CS) are scarce. Therefore we have studied the effect of alpha-toc (0.1-2.5 micromol/L), AA (10-50 micromol/L) and Se (1-50 nmol/L) on t-butyl hydroperoxide (t-BHP, 100-500 micromol/L)-induced cell death in SW1353 chondrocytes. Furthermore we have determined the effect of GS and CS alone (100-500 micromol/L each) and in combination on MMP3 mRNA levels and MMP3 secretion in IL-1beta stimulated chondrocytes. A combination of alpha-toc, AA, and Se was more potent in counteracting t-BHP-induced cytotoxicity as compared to the single compounds. Similarly a combination of CS and GS was more e...Continue Reading

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Citations

Mar 28, 2013·BMC Research Notes·Yves HenrotinUNKNOWN Participants at the Hohenheim Consensus Conference in August 29th 2011
Aug 25, 2015·Molecular Medicine Reports·Zhiqiang ChangPei Zhang
Jun 1, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Fazal-Ur-Rehman BhattiSheikh Riazuddin

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Methods Mentioned

BETA
ELISA
nuclear translocation
PCR
protein assay

Software Mentioned

SPSS

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