Synergistic cytotoxicity of cisplatin and Taxol in overcoming Taxol resistance through the inhibition of LDHA in oral squamous cell carcinoma

Oncology Letters
Lin FengHong-Chen Liu

Abstract

The development of chemoresistance in patients represents a major challenge in cancer treatment. Lactate dehydrogenase-A (LDHA) is one of the principle isoforms of LDH that is expressed in breast tissue, controlling the conversion of pyruvate to lactate and also playing a significant role in the metabolism of glucose. The aim of this study was to identify whether LDHA was involved in oral cancer cell resistance to Taxol and whether the downregulation of LDHA, as a result of cisplatin treatment, may overcome Taxol resistance in human oral squamous cells. The OECM-1 oral epidermal carcinoma cell line was used, which has been widely used as a model of oral cancer in previous studies. The role of LDHA in Taxol and cisplatin resistance were investigated and the synergistic cytotoxicity of cisplatin and/or Taxol in oral squamous cells was analyzed. Cell viability was analyzed by MTT assay, LDHA expression was analyzed by western blot analysis and siRNA tranfection was performed to knock down LDHA expression. The present study results showed that decreased levels of LDHA were responsible for the resistance of oral cancer cells to cisplatin (CDDP). CDDP treatments downregulated LDHA expression, and lower levels of LDHA were detected in...Continue Reading

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·A GonçalvesM A Jordan
Oct 25, 2003·Oncogene·George A OrrSusan Band Horwitz
Oct 7, 2004·Japanese Journal of Clinical Oncology·Guan-Cheng HuangYoung-Chau Liu
Jul 20, 2005·Journal of Clinical Pharmacology·Vera S Donnenberg, Albert D Donnenberg
Feb 3, 2007·Anti-cancer Agents in Medicinal Chemistry·Victoria CepedaJose M Pérez
Mar 19, 2008·Pathology Oncology Research : POR·Yukihiro HiraishiShigeyuki Fujita
Aug 30, 2008·Molecular Cancer Research : MCR·Xing-Jie LiangMichael M Gottesman
Nov 26, 2008·Nature Cell Biology·Allyson E Vaughn, Mohanish Deshmukh
Sep 6, 2011·Oncogene·L GalluzziG Kroemer
Dec 12, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Makoto ShimanishiHiroyoshi Hiratsuka

❮ Previous
Next ❯

Citations

Jun 19, 2018·International Journal of Radiation Biology·Michael I Koukourakis, Alexandra Giatromanolaki
May 18, 2016·Drug Development and Industrial Pharmacy·Huifeng DiChuanhai Dong

❮ Previous
Next ❯

Methods Mentioned

BETA
Transfection

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cancer Metabolism: Therapeutic Targets

Targeting the mechanisms by which cancer cells acquire energy for metabolic needs is a therapeutic target. Discover the latest research on cancer metabolism and therapeutic targets.