Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by vitro-produced bovine morulae and blastocysts

Biology of Reproduction
E S Lee, Y Fukui

Abstract

The effect of glycine and alanine on the development of 2- to 4-cell bovine embryos, and amino acid uptake by bovine morulae and blastocysts, were examined through the use of a chemically defined medium. Bovine embryos at 2- to 4-cell stages were prepared by in vitro maturation and fertilization and cultured in a synthetic oviduct fluid medium (SOFM) containing polyvinyl alcohol (PVA) instead of BSA in order to examine the effect of amino acids. Morulae or blastocysts obtained from culture in SOFM containing BSA were cultured for 10 h in SOFM containing PVA to determine amino acid uptake. The combination of essential and nonessential amino acids with or without glutamine improved development to the blastocyst stage over that observed in the control (34% or 32% vs. 19%, respectively; p < 0.05). The optimal supplemental concentrations were 5 mM for alanine and 10 mM for glycine. At these concentrations, development to blastocysts was enhanced by the addition of alanine or glycine independently (35% or 36% vs. 26%, respectively; p < 0.05); the combined addition of alanine and glycine greatly (p < 0.01) improved proportions of blastocysts (45% vs. 26%) and hatched blastocysts (10% vs. 2%) compared to those obtained in the control. ...Continue Reading

Citations

Aug 2, 2005·Theriogenology·Sarah E HarrisNicolas M Orsi
Mar 10, 2012·Reproduction, Fertility, and Development·Tomomi MitoHiroyoshi Hoshi
Apr 14, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·K BenderL Brennan
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Nicolas M Orsi, Henry J Leese
Feb 27, 2007·Current Opinion in Cell Biology·Takahiro NobukuniGeorge Thomas
Mar 22, 2013·Reproduction, Fertility, and Development·Satoko MatobaTrudee Fair
Mar 4, 2020·International Journal of Molecular Sciences·Beatriz Rodríguez-AlonsoDimitrios Rizos
Mar 27, 2003·Molecular Reproduction and Development·S Sirisathien, B G Brackett
Apr 25, 2001·Biology of Reproduction·A E CrosierC E Farin
Apr 25, 2000·Biology of Reproduction·A E CrosierC E Farin
Jan 27, 2021·Metabolomics : Official Journal of the Metabolomic Society·Naomi InoueHisashi Narahara

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