Abstract
Platelet activation mediated through the interaction between von Willebrand factor (vWF) and platelet glycoprotein (GP) Ib is known to occur under high shear rate. We have demonstrated that low concentration of epinephrine could reduce the threshold level of shear rate necessary to cause platelet activation with purified system devoid of the effect of plasma proteins other than vWF. Both the extent of platelet aggregation and [Ca2+]i were continuously measured with optically modified cone-and-plate viscometer. No aggregation with no change in [Ca2+]i occurred under shear rate less than 7,200 s-1 in the absence of exogeneously added epinephrine. Epinephrine enhanced platelet aggregation under moderate level of shear rate (7,200 s-1) in a dose dependent manner. Significant aggregation with rise in [Ca2+]i was demonstrated even under the low shear rate of 1,200 s-1 when exogeneously added low concentration of epinephrine (0.05 microM) which did not cause platelet activation by itself was present. Aggregation and rise in [Ca2+]i under low shear in the presence of epinephrine was abolished by monoclonal antibodies against A1 domain of vWF or GP Ib, like aggregation and the rise in [Ca2+]i occurred under high shear rate (10,800 s-1),...Continue Reading
References
Jun 1, 1977·British Journal of Haematology·P N WalshJ G White
Dec 1, 1992·Circulation·S GotoS Ogawa
Sep 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·S P RouxH R Baumgartner
Apr 1, 1991·The Journal of Clinical Investigation·Y IkedaI Itagaki
Mar 1, 1990·Circulation·J StronyB Adelman
Oct 1, 1989·Circulation·S N WillichR Schröder
Jul 15, 1988·Thrombosis Research·Y IkedaK Sakai
Jun 11, 1987·The New England Journal of Medicine·G H ToflerJ E Muller
Jul 1, 1983·The Journal of Clinical Investigation·Z M RuggeriR R Montgomery
Oct 6, 1995·The Journal of Biological Chemistry·S GotoZ M Ruggeri
Citations
Dec 13, 2000·Thrombosis Research·M FrancesconiR Deana
Dec 13, 2000·Thrombosis Research·M LiS Handa
Oct 5, 2001·Thrombosis Research·M LiS Goto
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·M V SeftonM B Gorbet
Dec 30, 2011·American Journal of Veterinary Research·Natali B BauerAndreas Moritz
Sep 1, 2012·Journal of Echocardiography·Daniel RobaeiMichael Feneley
Jul 16, 2005·Journal of Atherosclerosis and Thrombosis·Tsutomu Michibayashi
Jan 10, 2013·International Journal of Cardiology·Faiez ZannadChristopher M O'Connor
Mar 28, 2009·American Journal of Hypertension·Carla SalaCesare Cuspidi
Mar 25, 2004·Psychosomatic Medicine·Kelly F Harris, Karen A Matthews
Feb 9, 1999·Circulation·S GotoZ M Ruggeri
Dec 1, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Jong-Shyan WangYu-Wen Chen
Feb 5, 2019·Cardiovascular Research·Maria T K ZaldiviaKarlheinz Peter
Sep 16, 2006·Platelets·I SinghJ A Hawley
Jan 8, 2004·Annals of Medicine·Ali HamaadRobert J MacFadyen
Aug 17, 2002·Journal of Applied Physiology·Yves CadroyDaniel Riviere