Synergistic effect of fermentable and non-fermentable carbon sources enhances TAG accumulation in oleaginous yeast Rhodosporidium kratochvilovae HIMPA1

Bioresource Technology
Alok PatelParul A Pruthi

Abstract

Novel strategy for enhancing TAG accumulation by simultaneous utilization of fermentable and non-fermentable carbon sources as substrate for cultivation of oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 were undertaken in this investigation. The yeast strain showed direct correlation between the size of lipid bodies, visualized by BODIPY stain (493-515 nm) and TAG accumulation when examined on individual fermenting and non-fermenting carbon sources and their mixtures. Maximum TAG accumulation (μm) in glucose (2.38 ± 0.52), fructose (4.03 ± 0.38), sucrose (4.24 ± 0.45), glycerol (4.35 ± 0.54), xylulose (3.94 ± 0.12), and arabinose (2.98 ± 0.43) were observed. Synergistic effect of the above carbon sources (fermentable and non-fermentable) in equimolar concentration revealed maximum lipid droplet size of 5.35 ± 0.76 μm and cell size of 6.89 ± 0.97 μm. Total lipid content observed in mixed carbon sources was 9.26 g/l compared to glucose (6.2g/l). FAME profile revealed enhanced longer chain (C14:0-C24:0) fatty acids in mix carbon sources.

References

May 14, 1999·Current Opinion in Microbiology·J Stülke, W Hillen
Aug 19, 2009·FEMS Yeast Research·Bernard TurcotteNitnipa Soontorngun
Apr 6, 2011·Applied Microbiology and Biotechnology·Jose Manuel AgeitosTomas G Villa
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May 23, 2012·Bioresource Technology·Zhiwei GongZongbao K Zhao
Jan 10, 2014·Microbial Cell Factories·Katrin WeinhandlAndrea Camattari

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Citations

Nov 12, 2017·Applied Microbiology and Biotechnology·Sujit Sadashiv Jagtap, Christopher V Rao
Feb 22, 2017·World Journal of Microbiology & Biotechnology·Jingyang Xu, Dehua Liu
Mar 23, 2021·Frontiers in Microbiology·Mauricio Ramírez-CastrillónPatricia Valente
Apr 17, 2021·Biotechnology for Biofuels·Shahryar ShakeriMahmood Maleki
Sep 4, 2021·Frontiers in Microbiology·Anjani Devi ChintaguntaJesus Simal-Gandara

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