PMID: 2107107Jan 22, 1990Paper

Synergistic effect of glucocorticoids and androgens on the hormonal induction of tissue plasminogen activator activity and messenger ribonucleic acid levels in granulosa cells

Molecular and Cellular Endocrinology
X C JiaA J Hsueh

Abstract

Tissue-type plasminogen activator (tPA) is secreted by rat granulosa cells in response to treatment with activators of protein kinase A (follitropin, FSH), protein kinase C (gonadotropin-releasing hormone, GnRH) and tyrosine kinase (epidermal growth factor, EGF). Because steroid hormones have been shown to enhance the gonadotropin stimulation of ovarian differentiation, we investigated the effects of steroid hormones, alone or together with various kinase activators, on tPA activities and mRNA levels in cultured rat granulosa cells. Treatment of cells with dexamethasone (DEX; a glucocorticoid agonist) or R1881 (an androgen agonist) caused an increase in tPA secretion and mRNA levels. In addition, the stimulation of tPA activity and mRNA levels by FSH (50 ng/ml) was synergistically enhanced by cotreatment with DEX or R1881 in a time-dependent manner with 2.8- and 1.6-fold increase at 9 h after incubation as compared to cells treated with FSH alone. In contrast, treatment with diethylstilbestrol had no effect on tPA levels. Furthermore, tPA activity and mRNA levels induced by GnRH and EGF were also increased by cotreatment with DEX or R1881 as compared with cells treated with GnRH or EGF alone. Likewise, the stimulation of tPA mR...Continue Reading

References

Jul 1, 1978·The Journal of Experimental Medicine·A Granelli-Piperno, E Reich
Aug 1, 1987·The Journal of Endocrinology·C R HarlowN Jenkins
Nov 1, 1987·Acta Endocrinologica·W J de Greef, P van der Schoot
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Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 1, 1982·Steroids·J R SchreiberG F Erickson

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Citations

Oct 24, 1998·Journal of Reproductive Immunology·S G Hillier, M Tetsuka
Aug 8, 2002·Kidney International·Wolfgang EberhardtJosef Pfeilschifter
Apr 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·M Tetsuka, S G Hillier
Nov 1, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Masafumi TetsukaAsuka Onuma
Jul 1, 1993·Thrombosis Research·T NyM Ohlsson
Jan 1, 1995·Annals of the Rheumatic Diseases·J A Da Silva
Apr 19, 2020·Nature Reviews. Endocrinology·Karolien De BosscherLuc Brunsveld

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