Synergistic effect of mutations in invA and lpfC on the ability of Salmonella typhimurium to cause murine typhoid.

Infection and Immunity
A J BäumlerF Heffron

Abstract

Penetration of the intestinal mucosa at areas of Peyer's patches is an important first step for Salmonella typhimurium to produce lethal systemic disease in mice. However, mutations in genes that are important for intestinal invasion result in only moderately decreased virulence of S. typhimurium for mice. Here we report that combining mutations in invA and lpfC, two genes necessary for entry into Peyer's patches, results in a much stronger attenuation of S. typhimurium than inactivation of either of these genes alone. An S. typhimurium invA lpfC mutant was 150-fold attenuated by the oral route of infection but was fully virulent when the intestine was bypassed by intraperitoneal challenge of mice. During mixed-infection experiments, the S. typhimurium invA lpfC mutant showed a strong defect in colonizing Peyer's patches and mesenteric lymph nodes. These data suggest that mutations in invA and lpfC deactivate distinct pathways for intestinal penetration and colonization of Peyer's patches. While the inv-mediated pathway is widely distributed, the lpf operon is absent from many phylogenetic groups within the genus Salmonella. To investigate how acquisition of the lpf-mediated pathway for mucosal penetration contributed to evolut...Continue Reading

References

Dec 1, 1978·Infection and Immunity·A W HohmannD Rowley
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A LeeS Falkow
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C GinocchioJ E Galán
Jan 1, 1990·Microbial Pathogenesis·P A Gulig
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Galán, R Curtiss
May 1, 1974·The Journal of Experimental Medicine·P B Carter, F M Collins
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LiR K Selander
Oct 1, 1994·Infection and Immunity·F Garcia-del Portillo, B B Finlay
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B D JonesS Falkow
Jun 1, 1993·Journal of General Microbiology·E F BoydR K Selander
Jul 1, 1993·Journal of Bacteriology·I Behlau, S I Miller
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A J BäumlerF Heffron

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Citations

Feb 22, 2003·Infection and Immunity·Nita H SalzmanYvonne Paterson
Dec 28, 2007·Infection and Immunity·Melissa BarmanNita Salzman
Feb 21, 2018·Molecular Plant-microbe Interactions : MPMI·Thibault MeyerCéline Lavire
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·T L Norris, A J Bäumler
Jul 19, 2000·The Journal of Experimental Medicine·D M MonackS Falkow
Jul 17, 2003·Cellular Microbiology·Scott R Waterman, David W Holden
Jun 10, 2004·Molecular Microbiology·Javier Pizarro-Cerdá, Karsten Tedin
May 24, 2006·The Journal of Experimental Medicine·Maria Lara-TejeroJorge E Galán
May 7, 2016·Journal of Internal Medicine·P BergmanJ Münch
Dec 24, 2009·Infection and Immunity·Cristiano G MoreiraVanessa Sperandio
Feb 27, 1999·Molecular Microbiology·B M AhmerF Heffron
Oct 12, 2013·Applied Microbiology and Biotechnology·Elodie Barbau-PiednoirNadine Botteldoorn
Sep 24, 1998·Infection and Immunity·A J BäumlerL G Adams
Oct 4, 2007·The Journal of Biological Chemistry·Yao-Hui SunRenée M Tsolis

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